FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565256
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTACCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGGCTGCACCAT28550.5050808837057899No Hit
GTATTAAGTTGCTGACAGTAATAAGTTGCAATATCTTCAGGCTGCAGGCTACTGATTGTGAGAGTGAACTCCGTT23720.419632874308278No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20660.36549811059059967No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18280.3233932943657387No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14590.2581131381179501No Hit
GCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13570.24006821687872398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13540.2395374839010997No Hit
GTCTCCATCCTTCCTGTCTGGTTTTGTAGGAGACAGCGTCAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAA13050.2308688452665695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12570.22237711762458073No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11750.20787041623618324No Hit
GTATTACTTGCCGAGTCAGTCAGGCCATTGACAACAATTTAGTCTGGTAT11630.20574748432568607No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11020.19495591378065866No Hit
AGTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTC10540.1864641861386699No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10370.18345669926546557No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9890.1749649716234768No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9750.17248821772789674No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9340.165234867033698No Hit
CAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT9340.165234867033698No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9190.16258120214557653No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9050.16010444824999648No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9030.1597506262649136No Hit
GGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCT8960.15851224931712357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8860.15674313939170925No Hit
GTATACGGGTAAGTATTAAGTTGCTGACAGTAATAAGTTGCAATATCTTC8790.15550476244391923No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8320.1471899457944719No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8240.14577465785414043No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC7660.13551382028673734No Hit
GTAGGAGACAGCGTCAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAA7490.132506333413533No Hit
GTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCCCCTGGCCAAAAGTA7470.1321525114284501No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7370.1303834015030358No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7340.1298526685254115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7300.12914502455524576No Hit
GAGTTCACTCTCACAATCAGTAGCCTGCAGCCTGAAGATATTGCAACTTATTACTGTCAGCAACTTAATACTTAC7090.12542989371187568No Hit
GGCCAAAAGTATACGGGTAAGTATTAAGTTGCTGACAGTAATAAGTTGCA7050.12472224974170995No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7010.12401460577154422No Hit
CAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAACAATTTAGTCTGGTATCAGCAAAAAGCAGGGAAGGCCC6950.12295313981629562No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6930.12259931783121275No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6920.12242240683867132No Hit
CAGTAATAAGTTGCAATATCTTCAGGCTGCAGGCTACTGATTGTGAGAGTGAACTCCGTTCCAGCTCCACGGCCG6920.12242240683867132No Hit
ACTTATTACTGTCAGCAACTTAATACTTACCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAA6710.11870727599530123No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT6550.11587670011463834No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6540.11569978912209689No Hit
GTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCC6480.1146383231668483No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6410.11339994621905826No Hit
GTATTACTGGACGTGGATCCGCCAGTCACCCGGGAAAGGTCTGGAGTGGATTGGGGAGGTCGAGGAAACTGGAGG6350.11233848026380967No Hit
CTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGGCTGCACCA6320.11180774728618537No Hit
CCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGG6250.11056937033839535No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6180.10933099339060531No Hit
CTCACAATCAGTAGCCTGCAGCCTGAAGATATTGCAACTTATTACTGTCA6180.10933099339060531No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGG6170.10915408239806389No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5880.10402366361436234No Hit
CCTTGGTCCCCTGGCCAAAAGTATACGGGTAAGTATTAAGTTGCTGACAGTAATAAGTTGCAATATCTTCAGG5830.10313910865165517No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5720.10119308773369942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)