FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565256
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTACCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGGCTGCACCAT26650.47146779512291775No Hit
GTATTAAGTTGCTGACAGTAATAAGTTGCAATATCTTCAGGCTGCAGGCTACTGATTGTGAGAGTGAACTCCGTT23830.42157889522623376No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20570.3639059116577268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15390.27226601752126467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14700.2600591590359059No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14470.2559902062074529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12680.2243231385425365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11700.2069858612734761No Hit
GTCTCCATCCTTCCTGTCTGGTTTTGTAGGAGACAGCGTCAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAA11640.20592439531822748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11420.202032353482316No Hit
GTATTACTTGCCGAGTCAGTCAGGCCATTGACAACAATTTAGTCTGGTAT10870.1923022488925372No Hit
GCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10440.1846950762132556No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9620.1701883748248581No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9390.16611942199640517No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9210.16293502413065938No Hit
AGTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTC9210.16293502413065938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9070.16045827023507933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9040.15992753725745504No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.15674313939170925No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8590.15196654259309056No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8570.1516127206080077No Hit
GTATACGGGTAAGTATTAAGTTGCTGACAGTAATAAGTTGCAATATCTTC8540.1510819876303834No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8300.146836123809389No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8140.14400554792872608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7990.14135188304060461No Hit
GAGTTCACTCTCACAATCAGTAGCCTGCAGCCTGAAGATATTGCAACTTATTACTGTCAGCAACTTAATACTTAC7770.1374598412046931No Hit
GTGCAGCCACAGTTCGTTTGATATCCACCTTGGTCCCCTGGCCAAAAGTA7360.13020649051049435No Hit
CAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT7350.13002957951795294No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7340.1298526685254115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7280.1287912025701629No Hit
GGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCT7180.12702209264474856No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7040.12454533874916851No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6940.1227762288237542No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6760.1195918309580084No Hit
GTATTACTGGACGTGGATCCGCCAGTCACCCGGGAAAGGTCTGGAGTGGATTGGGGAGGTCGAGGAAACTGGAGG6610.11693816606988693No Hit
GTAGGAGACAGCGTCAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAA6530.11552287812955546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6360.11251539125635111No Hit
CAGTATTACTTGCCGAGTCAGTCAGGCCATTGACAACAATTTAGTCTGGTATCAGCAAAAAGCAGGGAAGGCCC6240.11039245934585391No Hit
CAGTAATAAGTTGCAATATCTTCAGGCTGCAGGCTACTGATTGTGAGAGTGAACTCCGTTCCAGCTCCACGGCCG6190.10950790438314674No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6070.10738497247264955No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6020.1065004175099424No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGCCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT5990.1059696845323181No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC5970.10561586254723522No Hit
ACTTATTACTGTCAGCAACTTAATACTTACCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAA5970.10561586254723522No Hit
CTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGGCTGCACCA5940.10508512956961093No Hit
CCCGTATACTTTTGGCCAGGGGACCAAGGTGGATATCAAACGAACTGTGG5910.10455439659198663No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5860.10366984162927947No Hit
CCCCTAATCTCCTGATTTTTGGTGGGTCCATTTTGCAAGCTGGGGTCCCG5840.10331601964419662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)