FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242406
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45060.3626833740339309No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35990.2896798631043314No Hit
CTATTACTGTCAGCAGTATGCTGGCTCACCGGGAACTTTCGGCGGAGGGA35510.2858163917431178No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32090.2582891582944706No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30040.24178891602262062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28520.2295545900454441No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28480.22923263409867625No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGCGCCTGAAGATT28250.2273813874047614No Hit
CAGTAATAGACTGCAAAATCTTCAGGCGCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27310.21981542265571802No Hit
GTCTATTACTGTCAGCAGTATGCTGGCTCACCGGGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA27290.21965444468233414No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCATCTACTTAGCCTGGTACC25510.20532740505116687No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT25430.20468349315763126No Hit
GTAATAGACTGCAAAATCTTCAGGCGCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25430.20468349315763126No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25190.20275175747702442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24070.19373699096752592No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG24040.19349552400745007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22520.18126119803027352No Hit
CATCTATGGTGGATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20930.16846344914625333No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT20660.16629024650557064No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20550.1654048676519592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20450.16459997778503968No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC20070.16154139629074554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19750.1589657487166031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19730.1588047707432192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19500.15695352404930435No Hit
GTGTTAGCAGCATCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGGAT19180.1543778764751619No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19130.15397543154170215No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17720.14262648441813708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17170.13819959015007977No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17160.1381191011633878No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17060.1373142112964683No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16940.1363483434561649No Hit
GACTTCACTCTCACCATCAGCAGACTGGCGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATGCTGGCTCA16740.13473856372232587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16270.1309555813478042No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA15780.12701162099989857No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15640.12588477518621127No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGATGCTGCTAACACTCTGA15460.12443597342575614No Hit
GTCAGAGTGTTAGCAGCATCTACTTAGCCTGGTACCAGCAGAAACCTGGC15410.1240335284922964No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14870.11968712321093104No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14540.11703098665009667No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14520.11687000867671277No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13980.11252360339534741No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC13910.11196018048850376No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13710.11035040075466475No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13570.10922355494097742No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13350.10745279723375452No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13180.10608448445999134No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13040.10495763864630403No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12950.10423323776607646No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12810.10310639195238916No Hit
GTATGCTGGCTCACCGGGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12680.1020600351253938No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12660.10189905715200988No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12590.10133563424516623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA200.00573700955.11259567
TCGTATG800.047.45357540
GGTATCA11950.042.8177951
CGTATGC1009.094947E-1238.0155141
GTATCAA32300.036.3292851
TACGTAT1557.2259354E-834.42326470
ATCTCGT1003.929017E-1034.396837
TATCAAC35750.032.8180962
ATCAACG36150.032.5493053
TCAACGC36200.032.5017364
CTTATAC2000.032.4059331
CCCTAAG1900.032.37482528
CAACGCA37200.031.6267595
GCCGTCT1001.3345925E-831.3156546
AACGCAG37700.031.297766
ACGAGAC1700.030.09838922
CGCAGAG40100.029.3348148
ACGCAGA40200.029.2653757
AGAGTAC40750.028.95056711
GTCATAG3700.028.5799121
Produced by FastQC (version 0.11.5)