FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC117070.7414698038053292No Hit
GTCCAATATAGGTGCCTCCATATGAGCAGCAGTAATAGTCAGCCTCATCG114660.7262059255515422No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61120.38710715305869753No Hit
GTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG57270.36272294920928677No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT53690.34004880640905544No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGCCTCCATATGAGCAG51230.32446825018319825No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50790.32168148402897984No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48630.3080009956355442No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGCCTCCATATGAGCAGCAGTAATAGT43430.2750664865402362No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40270.2550524387053951No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39460.24992225555785677No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36800.2330749874437184No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG36270.22971820093977358No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATAATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC35440.22446134660340705No Hit
GTAATAGTCAGCCTCATCGTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG34300.21724108884020493No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33510.21223757688149467No Hit
GACTATTACTGCTGCTCATATGGAGGCACCTATATTGGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT32500.2058406818456752No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31860.20178720380317577No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31760.20115384785903526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31150.19729037659977794No Hit
GCAGTAATAGTCAGCCTCATCGTCAGCCTGGAGGCCAGAGATGGTCAGGG31100.19697369862770767No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30390.19247687142430986No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30280.19178017988575527No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29870.18918342051477904No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28210.1786697118420461No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27950.1770229863872807No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27820.17619962365989797No Hit
CTATTACTGCTGCTCATATGGAGGCACCTATATTGGACTGTTCGGCGGAG27630.17499624736603098No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCATTCAGGGCACAGGATCCTGGG27360.17328618631685153No Hit
TCTCTGGCCTCCAGGCTGACGATGAGGCTGACTATTACTGCTGCTCATAT27120.1717661320509142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27040.1712594472956018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27030.17119611170118773No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTAATAATAACTTTGTCTCCTGGTACCAACAACA26580.1683460099525553No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26520.167965996386071No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26470.16764931841400071No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25220.15973236911224398No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25200.15960569792341586No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24430.15472885715353374No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24310.15396883002056508No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22800.14440515526404293No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCATTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22370.1416817247042386No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22250.14092169757126996No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21980.1392116365220905No Hit
ATATAGGTGCCTCCATATGAGCAGCAGTAATAGTCAGCCTCATCGTCAGC21810.13813493141705158No Hit
GTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21680.1373115686896689No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA21490.13610819239580188No Hit
GACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCT21190.13420812456338024No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCAC21090.13357476861923973No Hit
GCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19580.12401109386271758No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18890.11964093784814785No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18850.11938759547049163No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGC18640.1180575479877965No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18600.11780420561014028No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT18490.11710751407158569No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18050.11432074791736732No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17870.11318070721791434No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17740.11235734449053164No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17370.11001392749721164No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17060.10805052407037596No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17030.10786051728713382No Hit
GATATATACTGACGAACCAAAACCTCTCGCGCAATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTG16810.10646713421002463No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16580.10501041553850139No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16330.10342702567815004No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16210.1026669985451814No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16120.10209697819545491No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16110.10203364260104085No Hit
TCCTCAGCCTCCTCATTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCGGGGT15970.1011469442792441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.046.7052041
CGTATGC2300.045.06637641
ACGAGAC2850.043.20365522
TCGTATG2400.043.14167840
TCCTATC2700.041.96169333
GTGGTAT3300.040.4318471
CGAGACG3050.040.3770223
ATGCCGT2700.039.78920444
ACGGACT3200.039.5872227
GACGGAC3250.038.96582826
CTTATAC3750.037.4046441
CTATCTC2850.037.3890735
CGGACTC3500.037.18639828
GGATGGG9800.037.1760870
TATACAC4150.036.2679023
TGCCGTC2600.036.0377345
CTCGGTT750.002111681735.98277370
GTATGCC3000.035.73917442
GTATCAA36050.035.397821
ACTCCTA3500.035.2773331
Produced by FastQC (version 0.11.5)