FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578891
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC114550.7255092340129876No Hit
GTCCAATATAGGTGCCTCCATATGAGCAGCAGTAATAGTCAGCCTCATCG106510.6745874161040882No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59950.3796968885122532No Hit
GTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG59170.374756712147957No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54070.3424555589967895No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT53260.33732537584925115No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGCCTCCATATGAGCAG47910.30344083283773227No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46530.2947005208085929No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGCCTCCATATGAGCAGCAGTAATAGT41320.26170267611887077No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40450.2561924794048481No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39320.24903555723606No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38490.24377870289969353No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATAATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC36800.2330749874437184No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG36760.23282164506606218No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34910.22110456009946222No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33500.21217424128708062No Hit
GACTATTACTGCTGCTCATATGGAGGCACCTATATTGGACTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT33340.21116087177645576No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33290.2108441938043855No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32520.2059673530345033No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30570.1936169121237628No Hit
GTAATAGTCAGCCTCATCGTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG29910.18943676289243525No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCATTCAGGGCACAGGATCCTGGG28850.18272318988454556No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28810.18246984750688933No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28430.1800630949191553No Hit
GCAGTAATAGTCAGCCTCATCGTCAGCCTGGAGGCCAGAGATGGTCAGGG28260.17898638981411638No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27720.17556626771575745No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27270.17271616596712502No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27090.17157612526767205No Hit
CTATTACTGCTGCTCATATGGAGGCACCTATATTGGACTGTTCGGCGGAG26410.16726930484751637No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26100.16530590142068072No Hit
TCTCTGGCCTCCAGGCTGACGATGAGGCTGACTATTACTGCTGCTCATAT26080.1651792302318526No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25750.16308915561618884No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25580.16201245051114993No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTAATAATAACTTTGTCTCCTGGTACCAACAACA25490.16144243016142343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25010.15840232162954884No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24450.15485552834236183No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24020.1521320977825575No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23820.15086538589427642No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23790.15067537911103426No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23770.15054870792220618No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCATTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC23580.14934533162833913No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22620.14326511456458996No Hit
GTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21970.13914830092767644No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20670.13091467365384946No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCAC20300.12857125666052943No Hit
ATATAGGTGCCTCCATATGAGCAGCAGTAATAGTCAGCCTCATCGTCAGC20160.12768455833873268No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19990.12660785323369378No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA19840.12565781931748296No Hit
GCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19540.12375775148506134No Hit
GACTCAGCCTCGCTCAGTGTCGGGGTCTCCTGGACAGTCAGTCACCATCT19290.12217436162471No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19160.12135099889732731No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18520.11729752085482784No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18520.11729752085482784No Hit
GATATATACTGACGAACCAAAACCTCTCGCGCAATAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTG18390.11647415812744515No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18170.11508077505033595No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18100.1146374258894376No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18060.11438408351178138No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT17910.11343404959557057No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17550.11115396819666462No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17460.11058394784693812No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17060.10805052407037596No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17050.10798718847596193No Hit
GGCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16980.10754383931506355No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16970.10748050372064949No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16510.104567066377603No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15900.10070359511834572No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGTCCAATATAGGTGC15830.10026024595744736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT807.7287586E-752.25283470
GGTATCA14350.047.4015161
GTGGTAT3750.039.1947821
GTATCAA35850.036.6128161
CTCTGTT52500.035.1669970
TTACGAG400.002685489634.26404630
GAATACG2550.033.49119
GGATGGG9800.033.41337670
TGGTCAG52000.032.69151770
TGGTATC4400.032.6091842
TATCAAC40450.032.261772
ATACGTA2650.032.22728311
AATACGT2650.032.22728310
GTGCTAG2900.031.824151
AATGGTA6800.031.76152670
TACGTAC2600.031.5321612
TATACCA1101.1768861E-931.0553845
ATCAACG42250.030.9622733
ACGTACC2650.030.93329413
ACGCGCT450.00451866330.81036244
Produced by FastQC (version 0.11.5)