FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763981
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCAGCGGCGGTCACAGACCTCAGGTTCAGGGAGA78500.44501613112612887No Hit
GTCTATTACTGTGCGAGACGATACGTTTCCATGGGAATTTTTGATTTCTGGGGCCGAGGGACATTGGTCACCGT56160.3183707761024637No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA54920.3113412219292611No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53870.30538877686324284No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC52920.30000323132732154No Hit
ACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATCCATGCCAGAGCCCCAGGTCTGACAATAATAGTCAGC47810.2710346653393659No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42840.242859758693546No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG42480.2408189203851969No Hit
CTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATACTCACCTGCACT40410.22908410011218944No Hit
GTATCAACAACGACCAGAGAAGGTCCCTCGATTCTTAATGAAGGTTGATAGTGATGGGAGGCACGACAAGGGGGA40270.22829044077005367No Hit
GACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATCCATGCCAGAGCCCCAGGTCTGACAATAATAGTCAG39980.2266464321327724No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38990.22103412678481232No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37950.21513837167180372No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35870.20334686144578654No Hit
GTGTCTGGTGACTCCATCAGTAGTAGAGGTTATTACTGGGGCTGGATCCG35800.20295003177471868No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATC35210.1996053245471465No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33940.19240570051491485No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCGGGGATCCCGTCCCCCTTGTC32340.18333530803336318No Hit
CACCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCACATGGTATC30230.1713737279483169No Hit
GCACTAACGCCATCACATGGTATCAACAACGACCAGAGAAGGTCCCTCGA28790.1632103747149204No Hit
GTCATACTCACCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCAC26920.1526093535021069No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26880.1523825936900681No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25920.1469403582011371No Hit
GGTTGATAGTGATGGGAGGCACGACAAGGGGGACGGGATCCCCGATCGCT25920.1469403582011371No Hit
CCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCACATGGTATCAA25640.14535303951686554No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25160.14263192177240003No Hit
CACCTGCACAGTGTCTGGTGACTCCATCAGTAGTAGAGGTTATTACTGGG25090.14223509210133214No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTC24880.1410446030881285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24260.13752982600152722No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24250.13747313604851752No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23820.1350354680691005No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22830.1294231627211404No Hit
CAATAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC22440.1272122545537622No Hit
CTATTATTGTCAGACCTGGGGCTCTGGCATGGATGTCTTCGCAACTGGGACCAAGCTCACCGTCCTAGGTCAGCC22350.12670204497667492No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22210.12590838563453915No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22160.12562493586949067No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21860.12392423727919971No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGC21510.1219400889238603No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATACTCAC21080.11950242094444327No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21050.11933235108541418No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTATTGTCAGAC20910.11853869174327841No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20810.11797179221318144No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20700.11734820273007476No Hit
GTGATGGGAGGCACGACAAGGGGGACGGGATCCCCGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTAC20240.11474046489162865No Hit
GGTTATTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG20080.11383342564347347No Hit
CTATCAACCTTCATTAAGAATCGAGGGACCTTCTCTGGTCGTTGTTGATA19380.10986512893279464No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19340.10963836912075585No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19300.10941160930871703No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATA19180.10873132987260067No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18800.10657711165823215No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18530.10504648292697032No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT18450.10459296330289272No Hit
TTGTTGATACCATGTGATGGCGTTAGTGCTGTGTTCACTGCCCAGAGTGC18260.10351585419570847No Hit
CAGTAATAGACAGCCGTGTCAGCGGCGGTCACAGACCTCAGGTTCAGGGA17950.10175846565240781No Hit
CGGTCATACTCACCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATC17690.10028452687415568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA17550.069.1354270
TCGTATG2250.052.1151340
CGACGTA200.007755813751.06826830
CGTATGC2400.048.92419441
TCGACGT5600.046.78330229
CGACGTC5350.046.45462430
GACGTCA5750.044.44511831
CTCGACG6000.043.64332628
CCACGGA18450.043.29409869
ACGTCAT5550.041.802632
CGAGACT6450.041.57026323
AGACTCG6500.041.26921525
CACGAGA6550.040.40253421
CTTATAC7050.039.464331
TTATACA7050.039.463212
TATACAC7350.039.237313
CGAGCCC6700.038.9894115
GTCGTAT801.6789272E-938.725339
CCGTGTC17900.038.47456712
CGTTTGC459.9585865E-538.37191841
Produced by FastQC (version 0.11.5)