FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763981
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCAGCGGCGGTCACAGACCTCAGGTTCAGGGAGA75270.42670527630399646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56760.3217721732830456No Hit
GTCTATTACTGTGCGAGACGATACGTTTCCATGGGAATTTTTGATTTCTGGGGCCGAGGGACATTGGTCACCGT56000.3174637368543085No Hit
CTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATACTCACCTGCACT50940.28877862063140136No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48730.2762501410162581No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46330.2626445522939306No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44940.2547646488255826No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43010.24382348789471087No Hit
GTATCAACAACGACCAGAGAAGGTCCCTCGATTCTTAATGAAGGTTGATAGTGATGGGAGGCACGACAAGGGGGA40250.22817706086403425No Hit
ACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATCCATGCCAGAGCCCCAGGTCTGACAATAATAGTCAGC39310.22284820528112262No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39080.22154433636189957No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39030.2212608865968511No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38870.22035384734869595No Hit
GTGTCTGGTGACTCCATCAGTAGTAGAGGTTATTACTGGGGCTGGATCCG36610.20754191796850419No Hit
GACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATCCATGCCAGAGCCCCAGGTCTGACAATAATAGTCAG36090.2045940404119999No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATACTCAC34170.1937095694341379No Hit
GCCTTGGGCTGACCTAGGACGGTGAGCTTGGTCCCAGTTGCGAAGACATC33350.18906099328734266No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32790.18588635591879957No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30430.17250752700851085No Hit
CACCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCACATGGTATC27370.1551604013875433No Hit
GGTTGATAGTGATGGGAGGCACGACAAGGGGGACGGGATCCCCGATCGCT27310.1548202616694851No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26980.15294949322016507No Hit
GCACTAACGCCATCACATGGTATCAACAACGACCAGAGAAGGTCCCTCGA26650.15107872477084502No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26460.15000161566366077No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCGGGGATCCCGTCCCCCTTGTC26160.14830091707336984No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24850.1408745332290994No Hit
GTCATACTCACCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCAC24830.14076115332308No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24800.14059108346405091No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24530.13906045473278905No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGC24430.13849355520269208No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24300.13775658581356603No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCATA23370.1324844201836641No Hit
CACCTGCACAGTGTCTGGTGACTCCATCAGTAGTAGAGGTTATTACTGGG23280.13197421060657683No Hit
CCTGCACTCTGGGCAGTGAACACAGCACTAACGCCATCACATGGTATCAA22990.1303302019692956No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22710.12874288328502403No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAACCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT22580.128005913895898No Hit
CTATTATTGTCAGACCTGGGGCTCTGGCATGGATGTCTTCGCAACTGGGACCAAGCTCACCGTCCTAGGTCAGCC22060.12505803633939366No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGCTTGGTC21740.12324395784308335No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21360.12108973962871482No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGCCTCCAGTCTGACGATGAGGCTGACTATTATTGTCAGAC21310.12080628986366633No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20360.11542074432774503No Hit
CAATAATAGTCAGCCTCATCGTCAGACTGGAGGCTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC20240.11474046489162865No Hit
GGTTATTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG20120.11406018545551229No Hit
GTGATGGGAGGCACGACAAGGGGGACGGGATCCCCGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTAC19490.1104887184159013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19340.10963836912075585No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19240.10907146959065886No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18810.10663380161124185No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18560.10521655278599938No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18470.10470634320891212No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17880.10136163598133993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA17300.065.8676370
AATGGTA6500.044.38208470
CCTAACG551.6888771E-743.1416662
CCACGGA18600.041.75243469
GTGGTAT5400.040.1936231
CGTGTCA17450.034.76721613
CCGTGTC17550.034.37793712
CGGCGGT17550.033.81770721
AGCGGCG17750.033.43381519
GTGTCAG18700.032.62352414
CCGAGGG14000.032.03180354
GTCCACG20550.031.61426767
GACGTGT19600.031.60198862
AGACCTC18350.031.55173931
GCCGTGT19150.031.50562911
ACGTGTC19800.031.20711963
TCAACGC53850.031.032054
ATCAACG53750.030.9645373
TGTCCAC20550.030.9236266
AGCCGTG19500.030.76719510
Produced by FastQC (version 0.11.5)