FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458132
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40370.27686107979250163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39770.2727462259932571No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31650.21705853791014806No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30960.21232645604101685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26830.1840025457228838No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25920.17776168412736296No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25710.17632148529762737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24640.16898332935564134No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24600.168709005769025No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23350.16013639368726562No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22830.15657018706125372No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22230.1524553332620092No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22160.15197526698543068No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21080.14456853014679055No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20310.13928780110442676No Hit
GTCTCCATCCTCCCTGCCCGCATCTGTGGGAGACACAGTCACCATCACTT20200.13853341124123192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20090.1377790213780371No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19570.1342128147520252No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19510.13380132937210074No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19500.13373274847544667No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19230.13188106426578663No Hit
CAATAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGAAA19140.13126383619589996No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19140.13126383619589996No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18670.12804053405315843No Hit
ATAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGAAACA18660.12797195315650434No Hit
CCATTACCCCTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAA18400.1261888498434984No Hit
GAGTACCATTACCCCTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18130.12433716563383837No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17990.1233770330806813No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17930.12296554770075685No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17500.12001656914463162No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.11939934107474495No Hit
TTCTTGGAGGGGTAATGGTACTCTGTTGACAGTAGTAAGTCGCAAAATCC16620.113981450239073No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16610.11391286934241893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16550.11350138396249447No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16170.11089530988963962No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16070.11020950092309888No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15980.10959227285321219No Hit
GTCTATTATTGTGTGAGGGAAAGTGGATACAGCTTTGGTTCTCCCTACTT15860.10876930209336329No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15810.1084263976100929No Hit
CTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15700.10767200774689809No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15410.1056831617439299No Hit
CTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCT15400.10561458084727582No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15310.10499735277738914No Hit
CTCCTGTGCAGCCTCTGGCTTCACCTTCAGTGGCTATGCCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG15300.10492877188073507No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTT15290.104860190984081No Hit
ATGGTACTCTGTTGACAGTAGTAAGTCGCAAAATCCTCAGGTTGCAGACTACTGATGGTGAGAGTGAAATCTGTC15100.10355715394765358No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15070.10335141125769134No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14830.10170546973799353No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14790.10143114615137724No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14750.10115682256476094No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14730.10101966077145279No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14690.1007453371848365No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14660.10053959449487426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT352.2990182E-549.1915839
CGTATGC2750.046.4259841
TCGTATG2600.045.0916740
GTGGTAT3250.044.075211
AGACCGG3850.039.88308725
ACGAGAC3950.039.72906522
ATGCCGT3150.039.5890444
CACGAGA4000.039.23245221
TATCTCG2450.039.2283136
TATCGCG350.001382099139.22830636
GAGACCG4050.038.75208724
CCCACGA4200.038.16734319
GTATGCC3550.037.9512342
GCCGTCT2850.037.77509346
AAAACCG901.1177483E-437.62310470
GACCGGA4100.037.45890426
GAGCCTA4000.036.7696831
CTTATAC4550.036.729341
ATCTCCG4450.036.0181710
TATACAC4650.035.9406933
Produced by FastQC (version 0.11.5)