FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458132
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40270.2761752708259609No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33900.23248923965731497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32730.22446527474878816No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32650.22391662757555558No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31110.21335516949082797No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26960.18489409737938678No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26010.17837891219724963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23760.1629482104500827No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23720.16267388686346643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22280.1527982377452796No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21830.14971209739584618No Hit
GTCTCCATCCTCCCTGCCCGCATCTGTGGGAGACACAGTCACCATCACTT21350.14642021435645058No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21350.14642021435645058No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20420.14004219096762158No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20120.1379847640679993No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19920.13661314613491782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19270.13215538785240294No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19000.1303037036427429No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18490.12680607791338505No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18370.12598310715353617No Hit
GAGTACCATTACCCCTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18290.12543445998030356No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17850.12241690052752424No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17760.12179967245763758No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17470.11981082645466941No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17370.11912501748812866No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17250.11830204672827974No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17120.11741049507177675No Hit
ATAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGAAACA17090.11720475238181453No Hit
CAATAATAGACAGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGCAGAAA17080.11713617148516046No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16820.11535306817215452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16780.1150787445855382No Hit
CCATTACCCCTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAA16610.11391286934241893No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16390.11240408961602927No Hit
GTCTATTATTGTGTGAGGGAAAGTGGATACAGCTTTGGTTCTCCCTACTT16300.11178686154614259No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16250.11144395706287223No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16130.11062098630302332No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTT16060.11014092002644478No Hit
TTCTTGGAGGGGTAATGGTACTCTGTTGACAGTAGTAAGTCGCAAAATCC15560.10671187519374102No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15250.10458586739746469No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15040.10314566856772912No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15010.1029399258777669No Hit
CTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCT14930.10239127870453431No Hit
CTCCAAGAACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC14830.10170546973799353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA150.00248035768.1082841
TATTCGA200.0070443652.3257849
TACGTAT901.8052342E-646.20155770
TAACGGC702.3035682E-839.20292336
GTGGTAT3700.038.6560551
GTATCAA36400.038.2641331
CCGAAAC906.904884E-834.60690368
TTAACGG807.502422E-834.2824135
TATCAAC41850.032.7715072
CGAGTGA850.00341965532.61286570
ATCAACG42600.032.034773
TCAACGC43150.031.708584
CAACGCA44450.030.8577885
AACGCAG44850.030.7354076
AGTTTAT7500.029.4943562
CGCAGAG47550.028.847028
GGTACGT954.108182E-628.687368
CGGTACT500.006723323428.40440859
CGAAACC1253.9862243E-728.33596469
ACGGGTT854.9061673E-628.0407416
Produced by FastQC (version 0.11.5)