FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621280
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27260.4387715683749678No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22570.3632822559876384No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGA21350.3436453772856039No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCC19030.30630311614730876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18130.29181689415400464No Hit
GTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAA17070.2747553438063353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16000.25753283543651817No Hit
GGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT15920.25624517125933555No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14780.23789595673448363No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13980.22501931496265773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12860.20699201648210147No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG12370.1991050733968581No Hit
CGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAG12310.19813932526397113No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12240.1970126191089364No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11880.19121813031161475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11620.1870332217357713No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11610.18687226371362348No Hit
CTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCC11480.1847798094257018No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA11160.1796291527169714No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11150.17946819469482359No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10900.175444244141128No Hit
GTCCCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA10680.17190316765387587No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACTGGGAC10560.16997167138810199No Hit
AGGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT10430.16787921710018028No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10200.16417718259078032No Hit
GTCTGGGACCCCAGAGTCCCAGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA10140.16321143445789338No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9960.16031419005923256No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9430.1517834148853979No Hit
ATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCTCCGACCTTC9210.14824233839814577No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9120.14679371619881534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8900.1432526397115632No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG8770.1411601854236415No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8630.13890677311357197No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGGGACTGAGTCATCACAACATCC8330.13407803244913727No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA8270.13311228431625033No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA8230.13246845222765902No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACC8210.1321465361833634No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8070.12989312387329385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8050.1295712078289982No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC8010.1289273757404069No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT7960.12812258562966777No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7780.12522534123100695No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC7670.1234548029873809No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGGGA7630.12281097089878959No Hit
GTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT7600.12232809683234613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7440.11975276847798093No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7320.11782127221220706No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCCCCACTCTCC7300.1174993561679114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC7190.11572881792428534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7070.11379732165851146No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTC6990.11250965748132886No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6830.10993432912696369No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6750.10864666494978109No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6700.10784187483904198No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC6620.10655421066185938No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG6620.10655421066185938No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6620.10655421066185938No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6600.10623229461756376No Hit
GTTTCTAACTGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC6520.10494463044038115No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCCCCACT6460.1039788823074942No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6280.10108163790883337No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6250.10059876384238992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTG150.002226432669.9836745
TCTATAC252.9768032E-453.566053
TTTCGAG200.00679611852.7988147
AACGGGG353.5436032E-451.70502569
CGCGTAT408.5011743E-751.53359239
GGAACGG200.00813346550.45380828
TATACCG200.00828537150.2181745
ATACCGT200.00828537150.2181746
TATTCCG352.1683525E-549.6747943
ATCGCGT709.094947E-1248.82142337
AAACGGG250.00958630248.39728568
ATTCCGT305.97473E-446.5109544
TCGCGTT306.538333E-445.6680138
GCATCGC1350.045.38015735
TCGCTGC705.9480953E-1043.044152
CGACCGG705.966285E-1043.02336511
CGAGACT6700.042.98314723
TCGCGTA405.2175157E-542.81376338
ACGAGAC6650.042.79587622
CTTATAC6900.042.701011
Produced by FastQC (version 0.11.5)