FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621280
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54710.8806013391707442No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28310.4556721607004893No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCC22050.3549124388359516No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTACTTGA18730.30147437548287404No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18030.29020731393252636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17230.27733067216070045No Hit
GTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAA17200.276847798094257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16100.2591424156579964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15630.25157738861704865No Hit
GGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT14900.23982745300025754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14570.23451583826937933No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG13160.2118207571465362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11850.19073525624517126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11510.18526268349214525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11270.18139969096059747No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10790.17367370589750192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10770.17335178985320626No Hit
CTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCC10760.17319083183105846No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10460.16836209116662376No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.1653038887458151No Hit
GTCTGGGACCCCAGAGTCCCAGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA10250.16498197270151943No Hit
GTCCCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA10130.16305047643574558No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9920.15967035797064125No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA9890.1591874839041978No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9840.15838269379345868No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9810.1578998197270152No Hit
CGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAG9760.15709502961627608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9670.15564640741694566No Hit
AGGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT9530.15339299510687612No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9420.15162245686325007No Hit
ATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCTCCGACCTTC9380.15097862477465876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9230.14856425444244142No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACTGGGAC8810.14180401751223282No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG8790.14148210146793716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8600.1384238990471285No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC8180.1316636621169199No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACC8120.13069791398403297No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8010.1289273757404069No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC7980.12844450167396343No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTC7680.12361576100952872No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7630.12281097089878959No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7580.12200618078805048No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCTCCGACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA7540.12136234869945918No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7520.12104043265516354No Hit
GTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT7490.12055755858872007No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA7490.12055755858872007No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7460.12007468452227658No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGGGACTGAGTCATCACAACATCC7410.11926989441153747No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC7390.11894797836724182No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7230.11637265001287664No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGGGA7200.11588977594643318No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT7130.1147630697913984No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCCCCACTCTCC6820.10977337110481586No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6750.10864666494978109No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6670.10735900077259851No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6620.10655421066185938No Hit
GTTTCTAACTGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC6510.10478367241823332No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6480.10430079835178985No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA6470.10413984032964203No Hit
GGGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCTCCGACCTTCGGCCAAGGGA6280.10108163790883337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)