FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430995
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62720.4382964301063246No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50430.3524121328166765No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC49740.34759031303393795No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46380.3241101471353848No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTG46010.32152453362869887No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41400.28930918696431507No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39370.2751232534006059No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39230.2741449131548328No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGCCTGCAAATGAGCTGCAATAGTAGTCAG39200.27393526881645286No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37540.26233494875942964No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37260.2603782682678836No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGCCTG35640.24905747399536685No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTACAACTATGTCTCCTGGTATCAGTTGCAGCCAGGCAAAGC32800.22921114329539938No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT32690.22844244738800626No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG31340.21900845216090903No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACT29840.20852623524191208No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27610.19294267275567No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCAC27290.19070646647961734No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26580.18574488380462545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26010.18176164137540662No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTACA25910.18106282691414016No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25100.1754024297778818No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25090.17533254833175518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24980.17456385242436206No Hit
GACTACTATTGCAGCTCATTTGCAGGCAGCACCAGTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAG24900.1740048008553489No Hit
CCCCTGAGGGCCGCTGACTGACCTGATAAATGATGAGTTTGGGGGCTTTGCCTGGCTGCAACTGATACCAGGAGA24210.16918298107261032No Hit
CTACTATTGCAGCTCATTTGCAGGCAGCACCAGTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCC23790.16624796033529118No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGC23490.1641515169514918No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22040.1540187072631281No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21590.15087404218742903No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21280.14870771735750302No Hit
ATAGTAGTCAGCGTCATCTTCAGCCCGGAGCCAAGAGATGGTCAGGGAGG21220.14828842868074313No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGT19770.13815561899237944No Hit
GTAGTATGTGGCTGTGTCCACTGGGTCCACGTTGGTCATTGTGAGGACCA19580.13682787151597314No Hit
GTTTGGGGGCTTTGCCTGGCTGCAACTGATACCAGGAGACATAGTTGTAA18830.1315867630564747No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCAC18220.12732399484274928No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17780.12424921121317685No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17740.12396968542867026No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17660.12341063385965709No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17480.12215276782937746No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17470.12208288638325081No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17310.12096478324522449No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17300.12089490179909783No Hit
CCTCAGGGGTCTCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTTGGCTCCGGG16910.11816952540015863No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16800.11740082949276552No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16620.1161429634624859No Hit
GCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCA16320.1140465200786865No Hit
TTACAACTATGTCTCCTGGTATCAGTTGCAGCCAGGCAAAGCCCCCAAAC16310.11397663863255986No Hit
GACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCT15970.1116006694642539No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15790.11034280343397426No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15270.10670896823538867No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15100.10552098365123569No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14770.10321489592905635No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14770.10321489592905635No Hit
GCACCAGTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCCC14690.10265584436004319No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14550.10167750411427014No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCA14340.10020999374561058No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14310.10000034940723063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT252.616888E-454.98702237
CACGCAA200.006063926554.3464566
CGTATGC2000.048.29038241
TCGTATG1800.047.8614840
AATGGTA5850.046.88846670
CTCGACG4350.046.34184328
ACGAGAC4800.045.50385322
AGACTCG4750.045.28334425
ACTCGAC4550.045.03528227
TCGACGT4250.045.0346129
GACTCGA4550.045.0274126
CGACGTC4250.044.2598530
CACGAGA5100.042.82266621
TCTCGTA1550.042.16573338
CGAGACT5200.042.0050223
CTTATAC4850.041.5251051
TATACAC5100.041.4917033
GTGGTAT3700.040.5930251
TTATACA5000.040.273722
TGCCGTC2250.040.05111745
Produced by FastQC (version 0.11.5)