FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430995
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54620.38169245874374125No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48150.3364791630998012No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46200.3228522811051052No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTG45760.31977749747553275No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC41920.29294302216290063No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41430.28951883130269496No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40380.28218127945939714No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC40090.2801547175217244No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGCCTGCAAATGAGCTGCAATAGTAGTCAG39990.2794559030604579No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37950.2652000880506221No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37890.26478079937386223No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33090.2312377052330721No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGCCTG32650.22816292160349966No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACT30600.21383722514753722No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTACAACTATGTCTCCTGGTATCAGTTGCAGCCAGGCAAAGC30380.212299833332751No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCAC29920.20908528681092528No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29340.2050321629355798No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28770.20104892050636097No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27020.1888196674341979No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26810.18735215706553832No Hit
CCCCTGAGGGCCGCTGACTGACCTGATAAATGATGAGTTTGGGGGCTTTGCCTGGCTGCAACTGATACCAGGAGA25880.18085318257576022No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25840.18057365679125362No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25330.17700970303879468No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24790.1732361049479558No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24390.17044084710288995No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACACTGGTGCTGC23880.16687689335043102No Hit
GACTACTATTGCAGCTCATTTGCAGGCAGCACCAGTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAG23200.16212495501381907No Hit
CTACTATTGCAGCTCATTTGCAGGCAGCACCAGTGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCC22120.15457775883214128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21570.15073427929517572No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21470.15003546483390925No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTCGTTACA21380.14940653181876948No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20550.14360637179025784No Hit
ATAGTAGTCAGCGTCATCTTCAGCCCGGAGCCAAGAGATGGTCAGGGAGG20470.14304732022124467No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGT19720.1378062117617462No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19540.13654834573146657No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19250.13452178379379384No Hit
GTTTGGGGGCTTTGCCTGGCTGCAACTGATACCAGGAGACATAGTTGTAA19220.1343121394554139No Hit
CCTCAGGGGTCTCTGGTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTTGGCTCCGGG18060.12620589170472293No Hit
GTAGTATGTGGCTGTGTCCACTGGGTCCACGTTGGTCATTGTGAGGACCA17720.12382992253641696No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17370.12138407192198435No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17160.1199165615533248No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17140.11977679866107149No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17050.11914786564593167No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCAC16770.11719118515438559No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16620.1161429634624859No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16620.1161429634624859No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16570.11579355623185267No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16440.11488509743220626No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16260.11362723140192664No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15790.11034280343397426No Hit
CTCTTGGCTCCGGGCTGAAGATGACGCTGACTACTATTGCAGCTCATTTGCAGGCAGCACCAGTGTCTTCGGAA15610.10908493740369463No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15460.10803671571179493No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15320.1070583754660219No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15250.10656920534313538No Hit
GACTCAGCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCT15190.1061499166663755No Hit
GCCTCCCTCCGCGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCA14680.10258596291391653No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14600.10202691134490337No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14350.10027987519173721No Hit
TTACAACTATGTCTCCTGGTATCAGTTGCAGCCAGGCAAAGCCCCCAAAC14350.10027987519173721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)