FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334361
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40090.3004434332238427No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33540.25135626715708864No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32170.24108918051411873No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30880.23142163177730765No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28470.21336055235427293No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27000.20234404332860448No Hit
GTTTAGGGGCTTGGCCCGGATTCTGTTGAAACCAGGCCACCCAAGAATAA23190.1737910505477903No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22500.16862003610717038No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21500.16112581228018505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21490.1610508700419152No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20980.15722881589015267No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAA19460.14583759567313492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19350.14501323105216654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19150.14351438628676946No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19100.1431396750954202No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18810.14096635018559447No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17850.1337718953116885No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG17630.13212316606975172No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17290.12957512996857673No Hit
GGACACGACTGGAGCTTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17090.12807628520317965No Hit
CCCCATAGATGAGGAGTTTAGGGGCTTGGCCCGGATTCTGTTGAAACCAGGCCACCCAAGAATAAACAGTTTGA16530.12387951986006784No Hit
CCCCTAAACTCCTCATCTATGGGGCGTCTACTTTAGAAACTGGGGTCCCG16310.12223079061813107No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16050.12028229242311488No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15990.11983263899349576No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15280.11451174007633617No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15140.11346254874055821No Hit
TCTATGGGGCGTCTACTTTAGAAACTGGGGTCCCGTCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15030.11263818411958981No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14520.10881612996782729No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14270.10694257401108095No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14110.1057434981987633No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14090.10559361372222359No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13910.10424465343336624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13860.10386994224201697No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13510.1012469639025721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.042.0630461
CTAACGC1000.040.2932053
CCTAACG1050.038.3730432
CGAGACG5300.037.40695623
TCGTATG1950.037.0776840
GACGTAG5600.036.0517426
GAGACGT5650.035.6951624
GTATCAA49300.035.49271
CGTATGC2100.034.51548441
ACGTAGA5900.034.2417527
CGTAGAG5950.034.00000428
AGACGTA5950.033.9132225
ACGAGAC5900.033.5940422
AATCTCG2150.033.341636
CACGAGA6050.033.31140521
CTTATAC6650.031.8175811
CGAGCCC6500.031.5089915
GTGGTAT5250.031.3462091
TATCAAC56150.031.2144032
ATCTCCG6350.031.19347610
Produced by FastQC (version 0.11.5)