FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334361
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44520.3336428447773878No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36070.2703166534393616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33290.24948271120034232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31430.23554345488214956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29150.21845662455662299No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25590.19177718773255514No Hit
GTTTAGGGGCTTGGCCCGGATTCTGTTGAAACCAGGCCACCCAAGAATAA23790.17828758484398152No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22470.16839520939236083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22020.16502280867021743No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21830.1635989061430902No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21620.1620251191394233No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20120.15078378339894527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19740.14793597834469083No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19730.14786103610642098No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19680.14748632491507171No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19410.14546288448178565No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAA18950.1420155415213724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17770.13317235740552968No Hit
GGACACGACTGGAGCTTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17640.1321981083080216No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16790.12582801805508403No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16600.12440411552795683No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTAGACGGGACCCCA16390.1228303285242899No Hit
CCCCATAGATGAGGAGTTTAGGGGCTTGGCCCGGATTCTGTTGAAACCAGGCCACCCAAGAATAAACAGTTTGA16200.12140642599716268No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15590.11683494946270162No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15370.11518622022076484No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15190.11383725993190748No Hit
TCTATGGGGCGTCTACTTTAGAAACTGGGGTCCCGTCTAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14770.11068968592457364No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14460.10836647653820818No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14410.1079917653468589No Hit
CCCCTAAACTCCTCATCTATGGGGCGTCTACTTTAGAAACTGGGGTCCCG14280.10701751624935081No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14270.10694257401108095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14160.10611820939011257No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG13950.10454442238644565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG1100.042.6765142
CTAACGC1350.039.7351343
GGTATCA18950.038.2321171
GTATCAA47250.037.481411
CGAGACG400.002405732635.04083647
TATCAAC53850.032.5663872
TCAACGC55450.032.1044434
ATCAACG55600.032.019033
CAACGCA55900.031.9059735
AACGCAG56850.031.3751566
CGCTTAA450.004610555730.68344341
TGTTAGA16850.030.22173170
GTGGTAT6500.029.929431
TGGTATC6600.029.4671152
CGCAGAG60800.029.2816568
ACGCAGA61350.029.07387
AGAGTAC61850.028.7292711
CAGAGTA62800.028.34912110
GCAGAGT63650.028.1285659
AACGCCA1800.027.9377175
Produced by FastQC (version 0.11.5)