FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748432
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT90560.5179497973040988No Hit
GTCTACAATCCCCCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGA73700.4215205395462906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62160.35551854461597593No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC58960.33721643163703247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56840.3250912817884825No Hit
ATTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT56460.32291790587223296No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCAGTGAACCTGTCCGGGACCCCG52660.30118414670973764No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG52120.29809566514454094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49850.28511260375010294No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAATCC49150.28110901653595904No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGTAA47750.27310184210767136No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47470.2715004072220138No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGAAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC46700.26709646128645553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42260.24170227952817155No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG40280.23037784712245027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39070.22345736065228733No Hit
GTCCCGGACAGGTTCACTGGCAGTGGATCAGGCACAGATTTTACACTGAG38350.21933938523202504No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37870.21659406828518352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37660.21539299212094037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37030.21178976362821086No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36710.20995955233031652No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35770.20458330664275193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35030.20035094301637124No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34990.20012216660413445No Hit
GTCCGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA34250.19588980297775377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33990.19440275629821463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32920.18828298727088041No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31010.17735891358657357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30940.17695855486515918No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29990.17152511507453536No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29310.16763591606650988No Hit
GTTTATTACTGCATGCAAGGTCTACAATCCCCCACCTTCGGCCAAGGGAC28720.16426146398601718No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCC27700.15842766547397896No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA26270.15024890873651361No Hit
GATCTATATGGGTTCTAATCGGGCCTCCGGGGTCCCGGACAGGTTCACTG25890.1480755328202641No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25420.14538740997648178No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25360.1450442453581266No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA25140.14378597509082425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25010.14304245175105465No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24990.14292806354493626No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24970.14281367533881786No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24960.14275648123575868No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC24920.14252770482352187No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGA24510.14018274659809474No Hit
GCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24330.13915325274302917No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23760.1358931888686549No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23270.13309067781875417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23220.13280470730345817No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23150.13240434858204378No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22720.12994500215049826No Hit
TTATTACTGCATGCAAGGTCTACAATCCCCCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGG22690.12977341984132068No Hit
GGACACGACTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21780.12456875646293364No Hit
GAAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT21600.12353926260786807No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTCTCAGTGT21590.12348206850480888No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCGGACAGGTTCACTGGCAGT21280.12170905130997373No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20900.11953567539372421No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT20810.11902092846619143No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20760.11873495795089543No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20700.11839179333254024No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT20600.11781985230194826No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20550.11753388178665228No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20480.11713352306523789No Hit
GGGTCTCTGGGTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG20440.11690474665300109No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20390.1166187761377051No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20130.11513172945816594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG20040.11461698253063315No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19750.11295835354191641No Hit
AGTCAGAGCCTCCTGCAGAGAAATGGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG19470.11135691865625888No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG19130.10941231915224613No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18960.10844001940023976No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18900.10809685478188458No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18880.10798246657576617No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18870.10792527247270697No Hit
GGATACAACCATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAAT18870.10792527247270697No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18630.10655261399928621No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18180.10397887936162228No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTG17800.10180550344537277No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17700.10123356241478079No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17650.1009475918994848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.043.917521
GTGGTAT4900.039.576541
GCGACGT350.001412496839.05549231
TGGTATC5200.035.9620972
GTATCAA45000.035.00481
AATGGTA8350.032.556370
TATCAAC50850.030.6907422
ATCAACG51600.030.1753123
TACGTAT1403.592248E-529.87308970
TCAACGC52350.029.8720284
CAACGCA53050.029.4778635
AACGCAG53100.029.4501086
ACGCAGA56000.027.7428937
ATTCACT4550.027.648782
CGCAGAG56350.027.5705788
AGAGTAC56400.027.54534711
TCCCTAG2055.6947283E-727.20151170
CAGAGTA57750.026.90219910
TGTTAGA24050.026.0846170
ACTTTAT5400.024.5524276
Produced by FastQC (version 0.11.5)