FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444095
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT70360.4872255634151492No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA66140.458003109213729No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC60760.42074794248300834No Hit
ATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC56770.39311818128308734No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG55890.3870243993643077No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55070.38134610257635404No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGGC54200.3753215681793788No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC52400.3628570142546024No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGG52160.36119507373129883No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46570.32248570904268764No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG45290.3136220262517355No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44550.3084977096382163No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC44400.30745899681115163No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGGCTGCTGTCATAGGA42800.2963793933224615No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG39270.27193501812553883No Hit
GTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC38400.2659104837285636No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG33900.23474909891662255No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32870.22761660417077823No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31770.21999937677230375No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGG31630.2190299114670434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31470.21792195111817436No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29760.20608062488963677No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC28190.19520876396635956No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG28070.1943777937047078No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27820.19264660565959996No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT27430.18994595230923172No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC27130.18786852665510234No Hit
GTGCAGGATATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG26550.18385217039045215No Hit
GTGATGGCCAGGGAGACTGAGGTGCCAGACTTGGAGACAGAGAATCGGTC24730.1712491214220671No Hit
GCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCA24170.16737126020102555No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23690.16404737915441853No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22980.15913080510631225No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAACTGGGACCAAGGT22910.15864607245368206No Hit
ACCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGT22500.1558069240597052No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT22440.15539143892887933No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC21910.15172132027325072No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAA21270.14728947887777466No Hit
GGGCAGAGGGTCACCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG21230.14701248879055742No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21030.14562753835447112No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20980.14528130074544957No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGA20880.14458882552740646No Hit
TCCAGGAACAGCCCCCAAAGTCCTCATCTACGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCT20750.14368860774395037No Hit
GACATAACCCATCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCT20680.14320387509132018No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20600.14264989491688565No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20330.14078021182816922No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20110.1392567663484743No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAAGTCCTCAT20090.13911827130486568No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGACTGAGGTGCCAGACTTGGAGAC19860.13752557830336648No Hit
CCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAA19590.13565589521465No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19350.13399395469134648No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19090.13219351912443433No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19000.1315702914281955No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18890.13080856868834806No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT18570.12859264799061002No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18400.12741544011993672No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC18210.12609973720565476No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTGCACTGGGGACAG18180.1258919946402418No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC18080.12519951942219867No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17880.12381456898611241No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16680.1155048663695948No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGACTGAGGTGCC16440.11384292584629128No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16140.11176550019216186No Hit
CCCCTGAGGGCCGATTGTTGTTACCGTAGATGAGGACTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTA16070.11128076753953169No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15940.11038054975607561No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15840.10968807453803246No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15820.10954957949442384No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15800.10941108445081521No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15650.1083723716237505No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15650.1083723716237505No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGGCACCTCAG15500.10733365879668581No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15420.10677967862225131No Hit
GGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC15180.10511773809894777No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA15150.10490999553353486No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG15040.10414827279368741No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG14980.10373278766286152No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT14980.10373278766286152No Hit
GTCTCCAAGTCTGGCACCTCAGTCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGC14920.10331730253203564No Hit
GCCCAGTGATGGCCAGGGAGACTGAGGTGCCAGACTTGGAGACAGAGAAT14780.10234783722677523No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14760.10220934218316662No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTG14720.10193235209594936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)