FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444095
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62730.4343897042784582No Hit
ATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC58680.40634445794771124No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC57940.401220141334192No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA55480.3841852509703309No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC53950.3735903801342709No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC52890.3662501428230137No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG49810.3449219061072852No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49270.34118253992985226No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC47470.32871798600507585No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTG46800.3240784020441868No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGGC44260.30648953150589126No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC41770.2892468985766172No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGG40160.2780980475661227No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCCATCAGGCTGCTGTCATAGGA39210.271519532994713No Hit
GTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC39070.27055006768945256No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG39020.270203830080431No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG35210.2438205242729876No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34350.23786523739781662No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG33800.23405662369857938No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31670.21930690155426064No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29700.20566513975881087No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29600.20497266454076774No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC29300.20289523888663835No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGG28950.20047157562348736No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT27670.19160789283253524No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27600.19112316017990508No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26960.18669131878442902No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC26020.18018205173482355No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC25500.17658118060099925No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24480.16951793337695928No Hit
GTGCAGGATATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG24190.16750975524463418No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23710.16418587419802713No Hit
GCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCA23590.1633549039363754No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22870.1583690823664648No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAACTGGGACCAAGGT22860.15829983484466048No Hit
ACCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGT22600.15649939927774834No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT22310.15449122114542327No Hit
GTGATGGCCAGGGAGACTGAGGTGCCAGACTTGGAGACAGAGAATCGGTC21680.15012862727175152No Hit
GGGCAGAGGGTCACCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG21430.14839743922664367No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21140.14638926109431857No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT20970.14521205322364525No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20560.1423729048296684No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20490.1418881721770382No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20270.14036472669734332No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20230.14008773661012608No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAA20160.13960300395749586No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19880.1376640733469751No Hit
TCCAGGAACAGCCCCCAAAGTCCTCATCTACGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCT19860.13752557830336648No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19660.1361406278672802No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19290.1335784695605206No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC19200.1329552418642818No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAAGTCCTCAT19010.13163953894999983No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18960.13129330134097827No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT18880.13073932116654377No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTGCACTGGGGACAG18680.12935437073045747No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA18400.12741544011993672No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17960.12436854916054692No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17920.12409155907332967No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17890.12388381650791673No Hit
CCATATCCTGCACTGGGGACAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAA17740.12284510368085202No Hit
GACATAACCCATCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCT17250.11945197511244066No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGACTGAGGTGCCAGACTTGGAGAC17140.1186902523725932No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17100.11841326228537595No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC16920.11716680689289832No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC16690.1155741138913991No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTGCACTGGGGACAG16580.11481239115155167No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA16370.11335819319366108No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16320.11301195558463951No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16190.11211173780118343No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15880.10996506462524973No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGA15670.10851086666735914No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15630.1082338765801419No Hit
GTCCTCATCTACGGTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATT15590.10795688649292465No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG15140.10484074801173054No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG15130.10477150048992623No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGTCTCCAAGTCTGGCACCTCAG15100.10456375792451328No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATATCCTGCACTGGGGACAGCT15000.10387128270647014No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG14980.10373278766286152No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14960.10359429261925289No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG14950.10352504509744857No Hit
GGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGATGGGTTATGTCTTCGGAACTGGGAC14830.10269407483579682No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14750.1021400946613623No Hit
GGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC14580.10096288679068899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA305.4056465E-447.46948657
GGTATCA13100.046.052961
GTGGTAT3950.042.044371
TGGTATC4100.041.3156282
GTACGAC350.001474846638.7132843
GTATCAA34900.037.680371
TATCAAC39500.033.0211222
TCAACGC41100.031.7268354
ATCAACG41200.031.6542173
CAACGCA42350.030.8682255
AACGCAG42950.030.4370046
ACGCAGA45900.028.5545967
CGCAGAG45700.028.4572418
CGACGCT606.0334825E-428.23038923
AATGGTA5000.027.97222570
AGAGTAC46850.027.6854711
CAGAGTA47850.027.31920810
CCTTCGT500.00843542827.1143251
GCAGAGT48050.027.0654729
AAACCCG550.00979194326.29748262
Produced by FastQC (version 0.11.5)