FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851314
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGCTCACCGTACACTTTTGGCCAGGGGA32060.376594300105484No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT24870.29213662643865834No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24520.28802533495279065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.2430360595502952No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19100.2243590496573532No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16560.19452282001705598No Hit
GTGTATTACTGTCAGCAATATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA15600.18324613479867594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15330.18007456708100653No Hit
GTGTATTACTGTGCGAGAGACCCGAGCAGCATGGATGTCTGGGGCCAAGG14400.16915027827570087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14150.16621364150008106No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTGGCAGCAACTTCTTAGCCTGGTACC14100.1656263141449571No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13910.16339447019548603No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12990.15258764686120516No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT12960.15223525044813077No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA12650.1485938208463622No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG12600.14800649349123823No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACCTTCAGCAG12570.14765409707816388No Hit
GTCCCAGACCCACTGCCATTGAACCTGTCTGGGATGCCAGTGGCCCTGGT12180.14307294370819698No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11810.13872672128027966No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11580.13602501544670945No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGGCAG11560.13579008450465985No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11510.1352027571495359No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11330.13308837867108964No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGA11210.13167879301879212No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA11120.130621603779569No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10960.1287421562431723No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10580.12427846834423022No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10500.12333874457603188No Hit
GGCCAAAAGTGTACGGTGAGCTACCATATTGCTGACAGTAATACACTGCA10340.1214592970396352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10270.12063703874246165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10240.12028464232938729No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10190.11969731497426331No Hit
ATATAGGAGACTACGCACCGAAGTTCCAGGGCAGAGTCACGATTACCGCG9990.11734800555376747No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9890.11617335084351955No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9830.1154685580173708No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9810.11523362707532121No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9790.11499869613327163No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9490.11147473200252786No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9480.11135726653150307No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9350.10983021540818076No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9280.10900795711100723No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG9010.10583638939333784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8860.10407440732796594No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCATTGAACCTGTCTGGGATGCCA8810.10348707997284198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8760.10289975261771803No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA8690.10207749432054448No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8660.1017250979074701No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8640.1014901669654205No Hit
GTATTACTGTGCGAGAGACCCGAGCAGCATGGATGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATC8600.10102030508132136No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8560.10055044319722219No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8520.10008058131312302No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8520.10008058131312302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT300.00218552570.30784670
GGTATCA7200.043.606841
GTATCAA19350.036.684421
AGTATAT400.002742775334.1150632
AACTGTG15400.033.7842970
TATCAAC21600.033.0094832
CCGGTCC450.00423753131.21580755
ATCAACG22650.031.0273883
TCAACGC22650.030.8785844
AACGCAG23000.030.7107756
GTGCGTC450.004603100530.69235441
CAACGCA23000.030.560625
ACGCAGA24450.028.889487
ATACGTA1302.237357E-1028.87168111
CGCAGAG25100.028.1413468
AGAGTAC25550.027.91117711
GAATACG1353.4742698E-1027.8039939
TACGTAC1353.4924597E-1027.80072612
TGCGTCC500.007648724627.66259642
ATGGCAA2055.27014E-727.4372170
Produced by FastQC (version 0.11.5)