FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399607
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGGGCATACCTACTACAACCCGTCCCTCCAGAGTCGAGTCA16050.4016446158350579No Hit
ATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAAGA11830.29604086014509257No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11170.2795246329518752No Hit
GTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAGGCAGTA9490.23748332736914018No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9410.23548136043662898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8290.2074538233814723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7520.18818489165605207No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7480.18718390818979647No Hit
CTCCAATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGG7140.1786755487266239No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACT6720.16816522233094014No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6570.16441153433248168No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6560.16416128846591777No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6520.16316030499966216No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCATCAGCGTAGCAGC6390.15990710873433148No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6190.1549021914030535No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6150.1539012079367979No Hit
GTCTACTATTGTGCGAGGACTTATTACTATGAGGATAGTGGTTATAATCC6080.15214948687085061No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6010.1503977658049033No Hit
GTAATAAGTCCTCGCACAATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAAGAATTGCTG5950.14889629060551993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5720.14314063567455024No Hit
GTTTATTACTGTCATCAGCGTAGCAGCTTTGGCCAGGGGACCAAGCTGGA5550.13888645594296395No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5510.13788547247670838No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCCAATGCAGGGCCAGTCAGATTGTTAATAA5500.13763522661014446No Hit
CTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCAACCAACAGGGCCC5460.13663424314388886No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5450.13638399727732498No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5360.1341317844782499No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5310.13288055514543037No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGGGGCCCTGTT5160.12912686714697189No Hit
CAATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAA5130.1283761295472802No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5110.1278756378141524No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5080.1271249002144607No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5070.1268746543478968No Hit
ATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGT5010.1253731791485134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4850.12136924528349104No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTA4780.11961752421754374No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4750.11886678661785205No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4700.11761555728503255No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4660.11661457381877695No Hit
GACTTATTACTATGAGGATAGTGGTTATAATCCGCCTTTTGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC4630.11586383621908525No Hit
ATCTATTATACTGGGCATACCTACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCCGCAGACACGTCCACG4530.11336137755344627No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG4500.11261063995375456No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCCAATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTA4490.11236039408719066No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4480.11211014822062677No Hit
GCCTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATCTATTATACTGGG4470.11185990235406287No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTGCTACGCTGATGACAGTAATAAACTG4470.11185990235406287No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT4450.11135941062093506No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA4370.10935744368842387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4300.10760572262247657No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4250.10635449328965708No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC4230.1058540015565293No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4070.1018500676915069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGT200.00772049351.1199954
GGTATCA4100.047.3795051
TGCGGAT2800.043.46886470
CTTGCAA350.001359586139.35253539
GTATCAA10750.037.725761
CACGCAG6350.037.2698970
CGCTCAT655.934362E-736.7429328
TCCGGTG1852.0190782E-1036.55039670
AATGGTA2600.036.40981770
CGTTGAA400.002562576734.58658642
CGAGACG701.0691583E-634.07999823
ATCAACG12150.033.6592563
TATCAAC12150.033.378762
TCAACGC12400.032.980644
ACGGGAT1057.348717E-1032.4408916
CAACGCA12600.032.186665
CTTATAC851.3418503E-732.0752871
TGTTAGA3300.030.7355670
AACGCAG13100.030.6980116
ATCTCCG902.2248969E-730.27816810
Produced by FastQC (version 0.11.5)