FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399607
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGGGCATACCTACTACAACCCGTCCCTCCAGAGTCGAGTCA15540.38888207664029906No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12240.30630094067421243No Hit
ATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAAGA10140.2537493086957936No Hit
CTCCAATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGG7780.19469128418671344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7230.180927761525699No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACT7110.1779248111269322No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6980.17467161486160152No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA6950.17392087726190983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6800.17016718926345134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6670.16691399299812065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6630.16591300953186505No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6400.1601573546008954No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6030.15089825753803113No Hit
GTCTACTATTGTGCGAGGACTTATTACTATGAGGATAGTGGTTATAATCC6020.1506480116714672No Hit
GCCTAGAGCCTGAAGACTTTGCAGTTTATTACTGTCATCAGCGTAGCAGC6010.1503977658049033No Hit
GTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAGGCAGTA5980.1496470282052116No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5700.14264014394142244No Hit
GTTTATTACTGTCATCAGCGTAGCAGCTTTGGCCAGGGGACCAAGCTGGA5550.13888645594296395No Hit
CTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATCTATGATGCAACCAACAGGGCCC5520.13813571834327226No Hit
GTCTCCAGCCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCCAATGCAGGGCCAGTCAGATTGTTAATAA5320.1331308010119943No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5210.1303780964797914No Hit
GTAATAAGTCCTCGCACAATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAAGAATTGCTG5090.12737514608102463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5060.1266244084813329No Hit
GCCTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGACTATCTATTATACTGGG5010.1253731791485134No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGGGGCCCTGTT4940.12362145808256612No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4940.12362145808256612No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4930.12337121221600222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4890.12237022874974662No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT4850.12136924528349104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4830.12086875355036322No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4820.12061850768379932No Hit
GACTTATTACTATGAGGATAGTGGTTATAATCCGCCTTTTGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC4770.11936727835097982No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGACTTTGCAGTTTA4760.11911703248441595No Hit
ATCTATTATACTGGGCATACCTACTACAACCCGTCCCTCCAGAGTCGAGTCACCATATCCGCAGACACGTCCACG4650.11636432795221305No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4650.11636432795221305No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCCAATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTA4630.11586383621908525No Hit
ATGCAGGGCCAGTCAGATTGTTAATAACTATTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGT4600.11511309861939355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4580.11461260688626575No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4550.11386186928657406No Hit
GTAATAAACTGCAAAGTCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA4350.10885695195529607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4350.10885695195529607No Hit
CAATAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAAAAA4300.10760572262247657No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4260.10660473915622098No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC4200.10510326395683758No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4180.1046027722237098No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTGCTACGCTGATGACAGTAATAAACTG4150.10385203462401811No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG4060.101599821824943No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT4030.10084908422525131No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4020.10059883835868741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT300.002162322970.48286470
CCGTCTA150.002478295268.11339627
AAAACCG608.020217E-658.7357270
AGATTGC200.00626270153.89756463
GATTGTC200.00780685650.976347
ATAAACG200.007810720750.9699633
GGTATCA3550.047.9130861
TGCGGAT2400.044.05179270
CACGCAG6000.038.76557570
GTATCAA9500.037.9572531
GTATAAA451.0867357E-437.7981031
TCCATAT750.001700541537.59086270
CTTAATA501.4638048E-435.9317163
ATACATG400.002575621734.55077741
TAGTCAC400.002635654534.38885538
TCCTACC400.002776062834.0268226
GGCAGTA1603.6379788E-1233.08823469
GCGAATG450.003523014532.41113766
AATGGTA2655.456968E-1231.91676970
TCTCAAC450.003877023531.7836661
Produced by FastQC (version 0.11.5)