FastQCFastQC Report
Wed 8 May 2024
EGAF00004692789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782656
Total Bases58.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGG57790.7383831466186932No Hit
GTAATAATGAGCCTCATCCTCAGTGTGGAGCGCACTGATGGCCAGGGAGG56860.726500531523428No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31770.405925464060839No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGG31440.40170905225284165No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29090.3716830893777087No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26280.3357797039823371No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25380.3242803990514351No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGACCATTAAGA25080.32044729740780115No Hit
CCTCACTCACTGTGCAGTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTC24350.3111200834082917No Hit
AAGTAGTACTGTTAACTGGTATCAGCAGGTCCCAGGAACGGCCCCCAAAC22390.28607715266988304No Hit
GTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAG22210.28377729168370264No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21940.2803275002044321No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21620.27623885845122254No Hit
GTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGT21080.26933927549268133No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC20830.26614502412298635No Hit
GTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAT20430.26103422193147435No Hit
GGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCG19930.2546457191920844No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCC18740.23944108267233624No Hit
ACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGCACCTCGGCC18560.23714122168615584No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18140.23177487938506827No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18010.23011386867282688No Hit
GTGCAGTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCA17990.22985832856325128No Hit
GTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTC17520.2238531359882247No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17400.2223198953307711No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17100.21848679368713714No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16520.21107613050944476No Hit
CCATTAAGACTGTTATCCCATGTTGCACAGTAATAATGAGCCTCATCCTC16190.2068597187014474No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15980.2041765475509036No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15930.20353769727696458No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15930.20353769727696458No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15840.20238776678387438No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15540.19855466514024042No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15360.19625480415406002No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15080.19267724262000163No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGAC14850.18973853135988225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14820.18935522119551884No Hit
GGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACT14640.18705536020933847No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG14460.18475549922315807No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCT14400.18398887889443127No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14020.17913361681249487No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT13990.1787503066481315No Hit
ATAGTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCC13910.1777281462098291No Hit
GACTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGA13620.1740228146209829No Hit
GTACTACTTCCTCCGATGTTGGAACCGCTTCCAGAACAAGAGATGATGAC12710.16239573963529313No Hit
GTGTGGAGCGCACTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCC12690.16214019952571754No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGCGCTCCACACTGAGGATG12490.15958479842996157No Hit
TTATTACTGTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCG12390.15830709788208358No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC12330.1575404775533568No Hit
CTCCTGTATTGCCTCCGGATTCACGTTTGACATGCATCCCATGAGCTGGG12280.15690162727941778No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12220.15613500695069096No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT12070.154218456128874No Hit
CTGTTATCCCATGTTGCACAGTAATAATGAGCCTCATCCTCAGTGTGGAG12010.15345183580014718No Hit
CCCCTGAGTGGCGCTGACTATTACTATAGATGAGGAGTTTGGGGGCCGTT11900.15204636519748138No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11830.1511519748139668No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11820.151024204759179No Hit
ATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCACTG11800.1507686646496034No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT11760.1502575844304522No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11550.14757441327990842No Hit
ACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCC11490.14680779295118163No Hit
AGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTC11450.1462967127320304No Hit
GGCCATCAGTGCGCTCCACACTGAGGATGAGGCTCATTATTACTGTGCAA11410.1457856325128792No Hit
CCCCCGGACAGAGGGTCATCATCTCTTGTTCTGGAAGCGGTTCCAACATC11390.1455300924033036No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11210.14323023141712324No Hit
GTTTGGTCCCTCCGCCGAACACCGGACCATTAAGACTGTTATCCCATGTT11080.14156922070488184No Hit
CCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCATCATCTCTTGTTCTGG11060.14131368059530625No Hit
AGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGACAGAGG11030.14093037043094284No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10920.13952489982827704No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10750.13735280889688445No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGACCATTAAGAC10610.13556402812985527No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCC10560.13492517785591626No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC10510.13428632758197728No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10460.13364747730803828No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10440.13339193719846268No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10410.13300862703409927No Hit
CTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGATA10390.13275308692452367No Hit
GCTCCACACTGAGGATGAGGCTCATTATTACTGTGCAACATGGGATAACA10370.13249754681494808No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10100.12904775533567747No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10090.1289199852808897No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9960.1272589745686483No Hit
GTAGTACTGTTAACTGGTATCAGCAGGTCCCAGGAACGGCCCCCAAACTC9680.12368141303458992No Hit
GGATGAGGCTCATTATTACTGTGCAACATGGGATAACAGTCTTAATGGTC9680.12368141303458992No Hit
CAGTAATAATGAGCCTCATCCTCAGTGTGGAGCGCACTGATGGCCAGGGA9640.12317033281543871No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTC9570.1222759424319241No Hit
TAGTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCA9520.12163709215798511No Hit
GGTATCAGCAGGTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAAT9440.12061493171968272No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC9320.11908169106222913No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9300.11882615095265353No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9230.11793176056913893No Hit
ACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAACT9220.11780399051435113No Hit
TCCTCACCCTCCTCACTCACTGTGCAGTTCCAGTGTCCTGGGCCCAGTCT9030.11537635947338294No Hit
GGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGAC9000.11499304930901953No Hit
CATCTATAGTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTG8970.11460973914465614No Hit
CTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCCAGAGAAGCGGTC8970.11460973914465614No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8830.11282095837762696No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8780.11218210810368795No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGC8630.11026555728187097No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8620.11013778722708317No Hit
GTCTCAGTCAGGGCACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8540.10911562678878077No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8520.10886008667920516No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8470.10822123640526617No Hit
GTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGACAGAGGG8470.10822123640526617No Hit
GTTCCAACATCGGAGGAAGTAGTACTGTTAACTGGTATCAGCAGGTCCCA8460.10809346635047837No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA8410.10745461607653939No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT8390.10719907596696378No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG8320.10630468558344919No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8260.10553806525472238No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA8230.10515475509035897No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8220.10502698503557117No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8090.10336597432332979No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8040.1027271240493908No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTG7960.1017049636110884No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7900.10093834328236159No Hit
GTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACC7880.100682803172786No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG7870.10055503311799821No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT7860.10042726306321041No Hit
ATGTTGCACAGTAATAATGAGCCTCATCCTCAGTGTGGAGCGCACTGATG7840.10017172295363481No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGGTCAGGGACCCCTGAGTGGCGCTG7830.10004395289884702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)