FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782656
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATGAGCCTCATCCTCAGTGTGGAGCGCACTGATGGCCAGGGAGG47540.6074188404611988No Hit
CATTATTACTGTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGG44150.5641047918881348No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28030.358139463570202No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27960.3572450731866874No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGG27820.3554562924196582No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22290.2847994521220051No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22000.2810941205331589No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC20980.26806157494480337No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19390.24774613623354322No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19050.243401954370758No Hit
GTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18800.24020770300106306No Hit
CCTCACTCACTGTGCAGTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCC18750.23956885272712405No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18150.23190264943985608No Hit
GTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAT18000.22998609861803906No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17990.22985832856325128No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17790.2273029274674953No Hit
AAGTAGTACTGTTAACTGGTATCAGCAGGTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATAGTCAGCG17620.22513083653610272No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCC17410.2224476653855589No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16750.21401484176956417No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16280.2080096491945376No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGACCATTAAGA15780.2016211464551476No Hit
GTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAG15730.2009822961812086No Hit
GGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14730.18820529070242867No Hit
ACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGGCCATCAGTGCGCTCCACA14670.18743867037370185No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14670.18743867037370185No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14530.18564988960667267No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14470.18488326927794588No Hit
GTGCAGTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCA14360.18347779867528008No Hit
GTTCCAGTGTCCTGGGCCCAGTCTGTGCTGACTCAACCACCCTCAGCGTC14350.18335002862049227No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13900.1776003761550413No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13670.1746616648949219No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13660.17453389484013412No Hit
CCATTAAGACTGTTATCCCATGTTGCACAGTAATAATGAGCCTCATCCTC13490.1723618039087415No Hit
GGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGTTCCAGTGTCCTGGG13340.17044525308692451No Hit
CTCCTGTATTGCCTCCGGATTCACGTTTGACATGCATCCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13260.16942309264862213No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12890.16469560062147356No Hit
GTGTGGAGCGCACTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCC12490.15958479842996157No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12420.15869040804644696No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAG12090.1544739962384496No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11890.1519185951426936No Hit
CTGTTATCCCATGTTGCACAGTAATAATGAGCCTCATCCTCAGTGTGGAG11880.1517908250879058No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11710.1496187341565132No Hit
ATAGTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGG11570.14782995338948401No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11360.1451467822389402No Hit
GACTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGATACCAGTTAACAGTACTACTTCCTC11250.14374131163627443No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCA11190.14297469130754764No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCAGTGCGCTCCACACTGAGGATG11140.14233584103360863No Hit
GTACTACTTCCTCCGATGTTGGAACCGCTTCCAGAACAAGAGATGATGACCCTCTGTCCGGGGGTCCCAGACG11130.14220807097882082No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11000.14054706026657945No Hit
TTATTACTGTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCG10990.14041929021179164No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10530.13454186769155288No Hit
AGTCTGTGCTGACTCAACCACCCTCAGCGTCTGGGACCCCCGGACAGAGG10420.13313639708888708No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGAC10350.13224200670537248No Hit
CCCCTGAGTGGCGCTGACTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGATACCAGTTAA10230.1307087660479189No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTC9840.1257257339111947No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCA9780.12495911358246789No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9760.12470357347289229No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9740.12444803336331672No Hit
GGCCATCAGTGCGCTCCACACTGAGGATGAGGCTCATTATTACTGTGCAA9420.12035939161010713No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9380.11984831139095593No Hit
ACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGTTCCAGTGTC9380.11984831139095593No Hit
CTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGATA9350.11946500122659252No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9300.11882615095265353No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9230.11793176056913893No Hit
GTTTGGTCCCTCCGCCGAACACCGGACCATTAAGACTGTTATCCCATGTTGCACAGTAATAATGAGCCTCATCCT9150.11690960013083654No Hit
AGACAGGACTCAGGACAATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGTTC8970.11460973914465614No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8640.11039332733665877No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8630.11026555728187097No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8620.11013778722708317No Hit
ATCTCCACCATGGCCAGCCTCCCTCTCCTCCTCACCCTCCTCACTCACTG8400.10732684602175159No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGACCATTAAGAC8370.10694353585738818No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8350.10668799574781258No Hit
GGATGAGGCTCATTATTACTGTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACCAAA8330.10643245563823699No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8320.10630468558344919No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8280.10579360536429798No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8240.10528252514514679No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8190.10464367487120778No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC7970.1018327336658762No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7860.10042726306321041No Hit
TAGTAATAGTCAGCGCCACTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGGC7860.10042726306321041No Hit
GTGCAACATGGGATAACAGTCTTAATGGTCCGGTGTTCGGCGGAGGGACC7830.10004395289884702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1700.052.3252261
ACCCGTC200.00760361751.3222524
TTCGTGC200.007620930751.2927363
GCTTGCC603.10627E-739.9147531
TGAGTTA350.001402344739.11017225
GCCTATG350.001407642639.0801773
GCCAGAG18400.037.1761770
GTCCTAG22700.036.725870
GTATCAA18650.036.6891631
TATCAAC21000.032.4101942
GACAGGT751.780898E-631.9318051
ATCAACG21500.031.4913523
TCAACGC21600.031.3415534
GAGCAAA2757.2759576E-1231.09279670
CAACGCA21850.030.9829545
GTGGCCG5800.030.71307270
AACGCAG22300.030.6643836
GTCTATG450.0047772730.45992928
CGCAGAG24000.028.6347838
AAGAGGT1255.697228E-428.5017370
Produced by FastQC (version 0.11.5)