FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558389
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25170.4507610286019244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18720.3352501571485112No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13980.250363098126933No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13880.24857223190284908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12840.22994722317237626No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11020.1973534578940488No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10980.1966371114044152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10280.1841010478358277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9930.17783301605153395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9710.17389311035854932No Hit
GTGTTAGCACCTACGTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA9260.16583421235017165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8990.16099887354514505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8940.16010344043310307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8910.1595661805658779No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTCGCGAC8430.15097002269027507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8430.15097002269027507No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8160.14613468388524845No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8000.14326929792671417No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTAAATAAAGGCCAGTCGCGACGCTG7690.13771761263205398No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7630.13664309289760362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7560.1353894865407449No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7540.1350313132959281No Hit
GTTTATTACTGTCAGCAGCGTCGCGACTGGCCTTTATTTACTTTCGGCCC7500.13431496680629454No Hit
GTGTTGACACAGTCTCCAGCCACTTTGTCCTTGTCTCCAGGGGAAAGAGT7330.13127049422535186No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7310.13091232098053507No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCGGACCCACTGCCA7290.1305541477357183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7220.12930054137885955No Hit
GTCCGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT7070.12661424204273364No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6770.12124164337048186No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTCGCGACTGG6700.11998803701362314No Hit
GAGTGAAGTCTGTCCCGGACCCACTGCCACTGAACCTGGCTGGGATGCCA6670.11945077714639793No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC6250.11192913900524544No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6060.10852649317948598No Hit
GTCCCGGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGGT6020.1078101466898524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6020.1078101466898524No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6000.10745197344503564No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5790.10369115437445937No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5750.1029748078848258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCAA2900.042.12658370
GGTATCA8750.038.7483941
ACGTAAT459.723066E-538.52220542
TAACGTA451.00566416E-438.30222340
TTAACGT451.02214144E-438.1966839
GTATCAA21400.034.1966171
TAGCGTT502.1695136E-433.60834526
TTAGCGT502.1740951E-433.59627525
CTTAGCG400.002955862333.59627524
ACGTACC1950.032.6586113
ATACGTA2000.031.84499511
TACGTAC2000.031.84499512
CGTAATT553.1093866E-431.6089443
ATTGTCC450.00451076930.81776442
CGTTAAG553.7235403E-430.65212829
GGACTGT553.853181E-430.473686
AGGTTAC553.853181E-430.473687
AATGGCA2700.030.42784569
GAATACG2100.030.3285689
AATACGT2100.030.32856810
Produced by FastQC (version 0.11.5)