FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485255
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47570.32028170246859966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39700.26729416834146325No Hit
GTCTATCAGTTTCCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT34690.23356258689585288No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32980.22204941239046494No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31850.21444129122608577No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30250.20366873028537189No Hit
CTGATAGACTGCTGACAAGAGTAAGTTGCAAAATCTTCAGCCTGCAGGGAGCTGATGGTGAGAGTGAAGGCTGTC26140.17599671436891307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26050.17539075781599792No Hit
GCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTGCAACTTACTCTTGTCAGCAGTCTATCAGTTTC25940.17465014425132383No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25570.17215898953378378No Hit
GATATATGGTGCATCCCGTTTGCAAAATGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTTCA25410.1710817334397124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24810.16704202308694469No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTATTGGCAGTTACTTAGTCTGGTATCAGCAGAAA24740.16657072354578842No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24020.1617230711224672No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23790.16017451548723957No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22280.15000791109944084No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22220.14960394006416408No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21820.1469107998289856No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20700.13937000717048587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20560.13842740808817341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20510.13809076555877609No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20380.13721549498234312No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20340.13694618095882524No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20180.13586892486475388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20030.13485899727656195No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19990.1345896832530441No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC19800.13331044164133432No Hit
CCATATATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC19620.132098528535504No Hit
CCTGATATATGGTGCATCCCGTTTGCAAAATGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTT17690.11910412690076788No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTG17660.11890214138312949No Hit
ATCTGGGACAGCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTGCAACTTACTCTTGTCAGCAGT17640.11876748437137058No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT17380.11701694321850457No Hit
GAGTGAAGGCTGTCCCAGATCCACTGCCGCTGAACCTTGAGGGGACCCCA16930.1139871604539288No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCGGAGGGAAA16660.11216929079518331No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16560.1114960057363887No Hit
GTATTGGCAGTTACTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16400.11041874964231732No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACCTCAACTTCAGGGAGA16350.11008210711292No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15890.10698499584246476No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15420.10382055606613005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15420.10382055606613005No Hit
ACCTTGAGGGGACCCCATTTTGCAAACGGGATGCACCATATATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG15320.10314727100733544No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15160.10207001491326405No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14980.10085810180743374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)