FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485255
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47050.3167806201628676No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34340.2312060891900717No Hit
GTCTATCAGTTTCCCTCCGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT33990.22884959148429057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33160.22326132549629527No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31970.2152492332966393No Hit
GCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTGCAACTTACTCTTGTCAGCAGTCTATCAGTTTC29800.2006389475207961No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27900.18784653140369836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26780.18030573874519865No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26210.17646801391006933No Hit
CTGATAGACTGCTGACAAGAGTAAGTTGCAAAATCTTCAGCCTGCAGGGAGCTGATGGTGAGAGTGAAGGCTGTC25320.17047577688679721No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23810.16030917249899848No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22810.1535763219110523No Hit
GAGTGAAGGCTGTCCCAGATCCACTGCCGCTGAACCTTGAGGGGACCCCA22320.15027722512295869No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC21980.147988055923057No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTATTGGCAGTTACTTAGTCTGGTATCAGCAGAAA21930.14765141339365967No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21690.1460355292525526No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21660.1458335437349142No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21580.14529491568787853No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21550.14509293017024014No Hit
GATATATGGTGCATCCCGTTTGCAAAATGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTTCA21280.14327506051149466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21100.14206314740566434No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20960.14112054832335189No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20660.13910069314696802No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20600.13869672211169126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20160.13573426785299494No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20000.13465701175892356No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19150.1289340887591693No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18300.12321116575941506No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18230.12273986621825883No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17580.11836351333609381No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCGGAGGGAAA17250.11614167264207156No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT16540.11136134872462979No Hit
CCATATATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC16500.11109203470111194No Hit
CCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTG16270.1095434790658843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16220.109206836536487No Hit
ATCTGGGACAGCCTTCACTCTCACCATCAGCTCCCTGCAGGCTGAAGATTTTGCAACTTACTCTTGTCAGCAGT16080.10826423745417453No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.10732163837186208No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACCTCAACTTCAGGGAGA15600.10503246917196038No Hit
CCTGATATATGGTGCATCCCGTTTGCAAAATGGGGTCCCCTCAAGGTTCAGCGGCAGTGGATCTGGGACAGCCTT15530.10456116963080413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15300.10301261399557653No Hit
GTATTGGCAGTTACTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCT15130.10186802939562566No Hit
ACCTTGAGGGGACCCCATTTTGCAAACGGGATGCACCATATATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG14990.1009254303133132No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14920.10045413077215697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1955.456968E-1240.247170
ATGGCAA6050.035.37874270
AATGGTA10500.034.65432470
GTATCAA40050.034.5542641
TAGCAAT5650.032.8323170
GTGCTAG4400.031.4522321
TATCAAC45150.030.2610112
ATCAACG45350.030.193813
TCAACGC46050.029.512144
AACGCAG46600.029.383896
GTGGTAT5300.029.295591
CAACGCA46650.029.0622315
GGTACGT1702.7284841E-1128.16025268
GTACGTA1952.910383E-1127.96512869
CGCAGAG49150.027.9280138
AGAGTAC49750.027.65898311
GCAGAGT50450.027.2752139
CAGAGTA50250.027.24953810
AATGGCA5550.027.02035969
GGGTCGA500.00860099527.00653322
Produced by FastQC (version 0.11.5)