FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612851
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46380.28756531136478203No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45170.28006306844215617No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35100.2176270467637742No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33380.20696270145227302No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30780.19084217946977122No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28800.17856578196001988No Hit
GTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG26510.1643673222138933No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAACTTATGATGTTCACTGGTACCAGCAGCTTCCAGGAACAGC26490.16424331819864327No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25430.15767110539039253No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGCTCTTGTCATAGGA24890.15432299697864216No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24840.15401298694051715No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24530.15209092470414193No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22650.14043454727064064No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22040.1366524248055152No Hit
GTCCTGGGCCCAGTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG21010.1302662180201395No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20430.12667010157788908No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19710.12220595702888859No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19130.11860984058663819No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19030.11798982051038813No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18760.11631576630451294No Hit
AGTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGG18740.11619176228926294No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17910.11104559565638734No Hit
CAATAATCGTCCCTCAGGGGTCCCTGACCGGTTTTCTGGCTCCAAGTCTGGCGCCTCAGCCTCCCTGGCCATCA17900.11098359364876235No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17550.1088135233818871No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17350.10757348322938698No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17330.10744947921413694No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17190.10658145110738686No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG17150.10633344307688683No Hit
GATTATTACTGCCAGTCCTATGACAAGAGCCTGAGTGGTTCGGTGTTCGG16500.10230331258126138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC553.0468073E-949.2890363
GGTATCA22200.045.5108871
GTATCAA61750.040.026041
TACGTAT1106.3400003E-638.48726370
CCTAACG754.7059075E-836.1497762
TGGTATC6150.035.2680742
GTGGTAT6250.035.2602461
TATCAAC70050.035.0757332
ATCAACG72850.033.9094663
TCAACGC73700.033.564364
CAACGCA75700.032.635865
AACGCAG77300.032.0928656
GACGTGT2950.031.79954362
CGCAGAG81700.030.488938
ACGCAGA84150.029.5609787
AGAGTAC83850.029.38108411
CAGAGTA85000.029.10588510
GCAGAGT85250.029.1000399
ACGTGTC3100.028.01727763
CGTGTCT3150.027.68717264
Produced by FastQC (version 0.11.5)