FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038231
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48890.4708971317558424No Hit
GTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT45220.4355485436285374No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACCC39590.38132169045231745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39490.3803585136641075No Hit
GTACACACTGGCCCGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA37490.3610949778999086No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTA33810.3256500720937826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32820.31611462189050415No Hit
GGTACACACTGGCCCGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT31370.30214855846145994No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30200.29087939003940355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28280.2723863957057726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24920.24002365562191844No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24290.23395564185619577No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG24260.2336666888197328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22940.2209527552153615No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22810.2197006253906886No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21910.21103203429679906No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21780.20977990447212613No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21670.2087204100050952No Hit
AGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACC20990.20217080784526759No Hit
GGTCTAGTCAGAGCCTCGTACACAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC20490.19735492390421785No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20290.19542857032779798No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA19590.18868633281032834No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19550.18830106209504438No Hit
CTCGTACACAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCC19480.18762683834329738No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAATCGGGAC19450.18733788530683443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19210.18502626101513053No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19200.18492994333630955No Hit
AGGTACACACTGGCCCGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18730.1804030124317228No Hit
CAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAAT18700.18011405939525982No Hit
ATGTTGGGGTTTATTACTGCATGCAAGGTACACACTGGCCCGGGACTTTT17730.17077124454962336No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17500.16855593793674048No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17500.16855593793674048No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC17050.16422164238979572No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT16270.15670886344175816No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16160.1556493689747272No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16060.15468619218651725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15820.15237456789481338No Hit
CTCCATCACTGTGTACGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC15570.14996662592428853No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA15360.14794395466904764No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15350.14784763699022666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15270.14707709555965867No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC15160.14601760109262776No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14950.14399492983738688No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCCGGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14600.14062381107865204No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA14560.14023854036336805No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTACA14000.1348447503493924No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13840.13330366748825648No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC13780.1327257614153305No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13660.13156994926947857No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13580.13079940783891061No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12790.12319031121205204No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12620.12155291067209512No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT12570.12107132227799015No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12560.12097500459916917No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12490.12030078084742221No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12440.11981919245331724No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12020.11577384994283545No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11670.11240273118410064No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11610.11182482511117467No Hit
GTGTACGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA11520.11095796600178573No Hit
AGTCAGAGCCTCGTACACAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGG11480.11057269528650177No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11460.11038005992885976No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11340.10922424778300782No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11220.10806843563715589No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT11180.10768316492187192No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11150.10739421188540893No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11080.10671998813366199No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11010.106045764381915No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10930.10527522295134704No Hit
TTATTACTGCATGCAAGGTACACACTGGCCCGGGACTTTTGGCCAGGGGA10920.10517890527252606No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10810.10411941080549511No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10690.10296359865964318No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10410.10026670365265533No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGC200.006124364754.20924864
GGTATCA11000.045.7640881
CCTAACG405.4416152E-542.5132182
CTAACGC405.4431614E-542.511173
TCGTATG955.456968E-1239.909340
GTGGTAT2100.038.872971
GTATCAA28650.038.703371
TAACGGC557.723234E-637.39786536
ATCTCGT952.3283064E-1036.11020337
ACCGGGT500.002443160934.92918469
TATCAAC33500.032.6907582
TAACCGT652.0340933E-532.4671853
ATCAACG34050.032.3609283
TCAACGC34200.032.119554
TAGCTCG652.561948E-531.3898410
TTCGATC553.3070432E-431.28183239
CAACGCA35200.031.2070645
AACGCAG35800.030.7790376
CTTATAC2350.030.3953551
TGTTAGA12500.030.2505470
Produced by FastQC (version 0.11.5)