FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785069
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGG123280.690617561562046No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG104470.5852434835852284No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC90530.5071512641808243No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG83290.46659260790479246No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT73570.4121409312469154No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC67150.37617593493584844No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG66170.3706859510752806No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC59700.33444085354683767No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCACTCAGG56310.31544999100875093No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53810.3014449301399553No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT52350.2932659745925788No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT49100.27505939546314456No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC47440.26576003504626433No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46330.2595417880205191No Hit
CCCCTGAGGGCCGCCGATTATTTCTATAGATGATGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCATA45970.2575250592554125No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG42320.23707767038697106No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG42190.23634940722179368No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41590.23298819261328274No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG40730.22817045167441707No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40220.2253134192571828No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39660.22217628562257258No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37830.21192458106661424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37520.2101879535188836No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36640.20525817209306754No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36590.20497807087569167No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36080.20212103845845736No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC35540.19909594531079752No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35460.19864778336299604No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35340.19797554044129387No Hit
ATACCAGACCACTCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCC34730.19455830558930776No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG34540.1934939209632793No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34450.19298973877200265No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCACTCAGGC34080.1909169897634209No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33290.18649139052888153No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAACCTCCCTGGCCATCAGTGGGCTCCGGT32450.1817856900769662No Hit
GTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC31950.1789846779032071No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA31530.17663182767724944No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG30560.17119786406015677No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA29360.1644754348431349No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAC27500.15405566955675104No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGGCG27340.1531593456611481No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC27070.1516467990873182No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25940.1453165115746226No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG25490.1427956006182394No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC25480.14273958037476422No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25390.1422353981834876No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25170.1410029528270336No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25080.14049877063575694No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG24150.135288887992565No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24130.13517684750561462No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24020.13456062482738762No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23530.1318156328971037No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23190.1299109446189475No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23160.12974288388852195No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTTTGTATGCTGGTACCAGCAGCTCCCAGGAACGGCCCC23130.1295748231580964No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22900.12828635755816722No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22820.12783819561036577No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGT22780.12761411463646505No Hit
CTGATGGCCAGGGAGGTTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC22450.12576544660178401No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA22390.1254293251409329No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22240.1245890214888052No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC21990.12318851540192564No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC20820.11663414691532932No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCATACAAAATTACCT20650.11568180277625123No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20290.11366507401114466No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20220.11327293230681838No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG20170.11299283108944248No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT20140.11282477035901695No Hit
GCTTGGTCCCTCCGCCGAATACCAGACCACTCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCCT19980.11192844646341402No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19640.11002375818525782No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC19480.10912743428965492No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19430.108847333072279No Hit
CCTCAACCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGAT19180.10744682698539945No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19170.10739080674192426No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG19170.10739080674192426No Hit
GCTGGTACCAGCATACAAAATTACCTCCGATGTTGGAGCTGCTTCCAGAA19100.10699866503759799No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGGCGGAGGGAC19090.1069426447941228No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG18780.10520601724639214No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18550.10391755164646296No Hit
GTCTGGCACCTCAACCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG18470.10346938969866151No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18350.10279714677695932No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18340.10274112653348413No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT18190.1019008228813564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)