FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785069
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGG122630.6869762457361592No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC87320.4891687660252909No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC82430.4617748669659268No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG70500.39494271650003443No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT66040.3699576879101032No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG62140.3481097929547821No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT58770.3292309709036457No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG51200.2868236465929328No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48170.2698495128199526No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47820.26788880429832124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT47550.26637625772449136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46180.25870148436839135No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG46100.2582533224205899No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC45910.2571889377945614No Hit
CCCCTGAGGGCCGCCGATTATTTCTATAGATGATGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCATA45350.2540518041599512No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42990.24083102669980824No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41890.2346687999175382No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCACTCAGG41090.23018718043952363No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41050.22996309946562288No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC40740.22822647191789225No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG40710.2280584111874667No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40290.22570556096150907No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39630.22200822489214703No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA39290.22010353661399085No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAACCTCCCTGGCCATCAGTGGGCTCCGGT37560.21041203449278428No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36740.20581837452781937No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36280.20324144332796099No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36260.2031294028410106No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA36050.2019529777280318No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG35270.1975833987369676No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG34980.1959588116761873No Hit
GTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC32980.18475476298115087No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32760.18352231762469687No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31570.17685590865115017No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTTTGTATGCTGGTACCAGCAGCTCCCAGGAACGGCCCC31450.176183665729448No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31130.17439101793824216No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30430.17046960089497942No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC29100.16301890851278017No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG28800.16133830120852471No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC28130.1575849448956875No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGGCG27660.15495199345235394No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26500.1484536452092328No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26130.14638089620065106No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25840.14475630913987078No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25640.14363590427036715No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG25470.14268356013128902No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25310.14178723623568612No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25110.1406668313661825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24940.1397144872271044No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC24710.1384260216271752No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24550.13752969773157228No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGT24370.136521333349019No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGCATACAAAATTACCT23630.1323758353318555No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCACTCAGGC23490.131591551923203No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGAC23430.13125543046235189No Hit
ATACCAGACCACTCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCC22790.12767013487994022No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT22680.12705391220171322No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22210.12442096075837965No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAATTTT21880.12257229272369864No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21570.120835665175968No Hit
CCCAGGAACGGCCCCCAAACTCATCATCTATAGAAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG21560.12077964493249281No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG21560.12077964493249281No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21530.12061158420206726No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG21300.11932311860213807No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21020.11775455178483296No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20850.11680220764575487No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCTGGTATTCGGCGGAGGGAC20760.11629802545447823No Hit
CTGATGGCCAGGGAGGTTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20690.11590588375015194No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20610.1154577218023505No Hit
GTCTGGCACCTCAACCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG20480.11472945863717313No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC20450.11456139790674758No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20300.11372109425461985No Hit
GCTGGTACCAGCATACAAAATTACCTCCGATGTTGGAGCTGCTTCCAGAA20020.11215252743731474No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20000.11204048695036438No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19660.1101357986722082No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC19540.109463555750506No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19310.1081750901505768No Hit
CCTCAACCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGAT19250.10783896868972573No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19200.10755886747234981No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19050.10671856382022207No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18740.10498193627249143No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAACCTCCCTGGC18690.10470183505511552No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18120.10150868117703013No Hit
GCCCCCAAACTCATCATCTATAGAAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT18030.1010044989857535No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18010.10089245849880313No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17920.10038827630752649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAA258.963725E-488.014470
ACGCATA200.0077895651.0122425
GGTATCA17250.045.7359471
GTATCAA41150.036.4441221
GTGGTAT4900.034.7002561
TATCAAC46500.032.1581232
TGGTATC5300.032.0615922
ATCAACG48350.030.9233323
GTCCTAG61200.030.9200970
CGTGCGT950.00446624130.88224270
TCAACGC49000.030.374434
CAACGCA49550.030.0406465
AACGCAG50150.029.8167656
CTAATGG1306.057149E-428.20974370
CGCAGAG53000.028.0851698
ACGCAGA53150.028.0698457
AGAGTAC54100.027.70102111
CAGAGTA54450.027.46208810
TCGTTGA1653.6379788E-1227.17146741
GCAGAGT54850.027.1379039
Produced by FastQC (version 0.11.5)