FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585542
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55230.3483351434399089No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47550.2998974483173577No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT42840.27019151810548064No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA40340.25442403922444184No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34640.21847418737567342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33250.20970746911781585No Hit
CTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG33040.20838300089180858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32630.20579713435531824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32280.2035896873119728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32120.20258056866358634No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA31870.20100382077548246No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31190.1967150665198399No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31100.19614743728012252No Hit
CCCCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG30840.19450761947649448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30730.19381385040572877No Hit
GTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT27810.17539743507267547No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27200.17155017022570201No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT26410.16656764689929374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26400.1665045769837696No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25940.16360336086965846No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25010.157737858725912No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24720.15590883117571153No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGCAGAG24690.15571962142913906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24200.15262919556845547No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24040.15162007692006899No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC22950.14474545612793605No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22300.14064591161886597No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG21820.13761855567370654No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC21700.13686171668741667No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGATCACCTTC21630.13642022727874759No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21330.13452812981302292No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21290.1342758501509263No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21130.13326673150253981No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC21120.13320366158701566No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21000.13244682260072582No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCC20980.1323206827696775No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA20910.13187919336100842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20910.13187919336100842No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20820.13131156412129102No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20470.12910411707794558No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA20270.1278427187674625No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT20250.12771657893641417No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC20150.1270858797811726No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19470.12279712552553006No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA19430.12254484586343346No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG18920.11932828017170154No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18900.11920214034065323No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA18590.11724697295940442No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18570.11712083312835611No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT18220.11491338608501069No Hit
ATATATTACTGTGCGAAAGATCGAAGCCCGGACGGACGCTGGAACTTTGA17880.11276900895718939No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17700.11163375047775462No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17630.11119226106908553No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17590.11093998140698891No Hit
GAGTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGG17540.11062463182936813No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17500.11037235216727151No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17050.10753420596868453No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCGGCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG17030.10740806613763622No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16780.10583131824953233No Hit
GGATACAACTACTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAAT16590.10463298985457338No Hit
CAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA16540.10431764027695262No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16460.10381308095275937No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA16440.10368694112171106No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16440.10368694112171106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16380.10330852162856613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.10255168264227627No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16260.10255168264227627No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCT16220.10229940298017964No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATA16040.10116414450074486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG452.0538373E-645.3490072
TACGTAT803.969558E-544.8450270
GGTATCA12350.043.534991
TCTGGAG15750.042.36786770
CTAACGC504.242058E-640.8102383
GTATCAA31200.038.2825471
GTGGTAT3350.035.5526161
TAAAGCG400.00249415734.78437447
TATCAAC35250.033.577562
ACTGCGA651.7082193E-533.3080964
ATCAACG37100.031.9918583
TCAACGC37450.031.4184514
TTGCGTT450.004221264331.24203353
CAACGCA37950.031.008425
CAACCGA450.00437983531.00748849
AACGCAG38450.030.6061556
CGCAGAG40750.029.045628
AGAGTAC40900.028.93727111
CGAAATT500.007064787728.11782853
TGGTATC4250.028.0096782
Produced by FastQC (version 0.11.5)