FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135443
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29190.25708027615653095No Hit
GTTCTGATAGGACCGGCAGTAATAATCAGCCTCATCTTCAGACTGGAGAC26370.2322441549245537No Hit
TATCAGAACAGTGTCAATGGTCCTCTTTATGTGTTCGGAAGTGGGACCAA25780.22704794516325344No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTCCGGACGGTGATCTTGGTCCCA24660.21718395375197172No Hit
CTCTATGATGTATATTGGTACCAACAACTTCCAGGGACAGCCCCCAAACTCCTCATCTTTGATAACATCAATCGG24290.21392531373217324No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21590.1901460487228333No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20370.1794013437926871No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19160.16874471021442733No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18970.16707135452858488No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18770.165309927490856No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18140.15976143232201No Hit
GTGTCAATGGTCCTCTTTATGTGTTCGGAAGTGGGACCAAGATCACCGTCCGGAGTCAGCCCAAGGCCAACCCCA17410.15333222363429957No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17280.1521872960597758No Hit
GATTATTACTGCCGGTCCTATCAGAACAGTGTCAATGGTCCTCTTTATGT15600.13739130894285315No Hit
GGTCATCACTGGTCTCCAGTCTGAAGATGAGGCTGATTATTACTGCCGGTCCTATCAGAACAGTGTCAATGGTC15060.13263545594098514No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14870.1309621002551427No Hit
CTCCTGCACCGGGAACAGTTCGACCATCGGGACACTCTATGATGTATATTGGTACCAACAACTTCCAGGGACAGC14860.13087402890325628No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14210.12514939103063738No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14100.12418060615988649No Hit
GTAATAATCAGCCTCATCTTCAGACTGGAGACCAGTGATGACCAGGGAGG14010.1233879639929085No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13990.12321182128913562No Hit
GCCTTGGGCTGACTCCGGACGGTGATCTTGGTCCCACTTCCGAACACATA13900.1224191791221576No Hit
CCATTGACACTGTTCTGATAGGACCGGCAGTAATAATCAGCCTCATCTTC13360.11766332612028962No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12940.11396432934105895No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12870.11334782987785384No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12750.11229097365521651No Hit
GTGATGACCAGGGAGGCTGAGGTTGCAGACTTGGAGCCAGAAAATCGGGC12650.11141026013635207No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTCCGGACGGTGATCTTGGTCCCACTTCCGAACACATAAAGAGGAC12560.11061761796937407No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12020.10586176496750607No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11710.1031315530590263No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11630.10242698224393475No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11560.10181048278072964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)