FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345616
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTGTGGAAACTCACCGTACACTTTTGGCCAGGGGA35060.2605498151032687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31620.23498531527568045No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT28540.21209617008121187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25350.1883895554155123No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24480.18192411505213968No Hit
GTGTATTACTGTGCGAGAGGTGGGGCTGGGGATCATGGCTACTGGGGCCA24360.18103233017443313No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23030.17114838111318534No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCCACTGTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA22670.16847302648006565No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21420.15918360067062223No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20500.15234658327487188No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20260.15056301351945875No Hit
GGGCTACAGTGGGTCTCAACTATTAGTGAAAAAATTACAACACTTTACGC19460.14461778100141495No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19240.14298284205895292No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19050.14157084933591751No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCACAGTGGAGCCTGAAGATT18950.14082769527116204No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18560.1379293944186157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18490.13740918657328688No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18020.13391636246893615No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17890.13295026218475406No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTTCCTATGCCATGTGCTGGG17610.13086943080343874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17240.1281197607638435No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17010.1264105064149059No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16880.12544440613072377No Hit
GTGTATTACTGTCAGCAGTGTGGAAACTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA16880.12544440613072377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16710.12418104422063947No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16490.12254610527817744No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16320.12128274336809314No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGAT15960.11860738873497342No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15920.11831012710907124No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15740.1169724497925114No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15730.11689813438603583No Hit
GTATTACTGTGCGAGAGGTGGGGCTGGGGATCATGGCTACTGGGGCCAGG15470.11496593381767162No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15110.1122905791845519No Hit
CTGTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCACATGGCATAGGAAC15100.11221626377807636No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15040.11177037133922307No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTTCTTCGTCTGGTACC14890.11065564024208988No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14620.1086491242672501No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14440.10731144695069024No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14360.10671692369888586No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC14220.1056765080082282No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14130.10500766934994828No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14060.10448746150461945No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13910.10337273040748624No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13900.10329841500101068No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13670.10158916065207312No Hit
GGCCAAAAGTGTACGGTGAGTTTCCACACTGCTGACAGTAATACACCGCA13470.10010285252256217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)