FastQCFastQC Report
Wed 8 May 2024
EGAF00004692808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345616
Total Bases99.4 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTGTGGAAACTCACCGTACACTTTTGGCCAGGGGA38300.284628006801346No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34420.2557936290888337No Hit
GTGTATTACTGTGCGAGAGGTGGGGCTGGGGATCATGGCTACTGGGGCCA33540.24925387331898552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33220.246875780311768No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30890.22956029060296548No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT30600.22740514381517463No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCCACTGTGCTGATGGTGAGAGT25270.1877950321637079No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25240.1875720859442813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24660.18326179236869952No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC23710.17620182875352253No Hit
GGGCTACAGTGGGTCTCAACTATTAGTGAAAAAATTACAACACTTTACGC22660.1683987110735901No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA21880.16260210936849742No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCACAGTGGAGCCTGAAGATT21760.16171032449079084No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21110.15687982306988027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20820.15472467628208939No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20710.15390720681085837No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20680.15368426059143173No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20630.153312683559054No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20470.15212363705544524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19980.14848218213814343No Hit
GTATTACTGTGCGAGAGGTGGGGCTGGGGATCATGGCTACTGGGGCCAGG19730.14662429697625473No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19600.1456581966920726No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC19570.14543525047264597No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19070.14171948014886862No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA18980.14105064149058869No Hit
GTGTATTACTGTCAGCAGTGTGGAAACTCACCGTACACTTTTGGCCAGGG18820.13986159498697992No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18450.1371119249473847No Hit
GTAATACACGGCCGTGTCTTCGACACTCAGGCTGTTCATTTGCAGGTACA18390.1366660325085314No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18260.13569993222434928No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG18260.13569993222434928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18230.13547698600492264No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTTCCTATGCCATGTGCTGGG18130.1347338319401672No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17960.1334704700300829No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCACCTTCTTCGTCTGGTACC17200.1278224991379413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17070.12685639885375918No Hit
CTGTAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCACATGGCATAGGAAC16960.12603892938252817No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16840.1251471445048216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA16580.12321494393645735No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG16540.12291768231055517No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16230.1206139047098132No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGAT15620.11608066491480482No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15540.11548614166300045No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14860.11043269402266323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14650.10887207048667674No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14560.1082032318283968No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14140.10508198475642383No Hit
GTAATACACCGCAAAATCTTCAGGCTCCACTGTGCTGATGGTGAGAGTGA14140.10508198475642383No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14040.10433883069166835No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13910.10337273040748624No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13870.10307546878158405No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA13830.10277820715568185No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC13820.10270389174920631No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC13790.10248094552977967No Hit
GGCCAAAAGTGTACGGTGAGTTTCCACACTGCTGACAGTAATACACCGCA13690.1017377914650242No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13660.10151484524559755No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13550.10069737577436653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)