FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392094
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATTGTATGCAAGCAACACACTGGCCTCCCACTTTTGGCCAGGGGA17290.44096568679959397No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTA15750.40168939080934674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14730.3756752207378843No Hit
CAATAATAGACTCCAACATCCTCAGCCTCCACCCGGGTGATTTTGAGTGTGAAATCAGTGCCGGACCCACTGCCG14350.3659836671818492No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13020.33206322973572666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10430.2660076410248563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8460.21576458706330623No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8140.20760327880559254No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7990.2037776655597892No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7780.1984218070156646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7680.19587139818512908No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7360.18771008992741536No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7320.18668992639520116No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7120.1815891087341301No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG6930.17674333195611258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6870.17521308665779126No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC6690.17062235076282728No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6550.16705177840007754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6190.1578703066101496No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6180.15761526572709605No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6130.1563400613118283No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCCGGCACTGATTTCACACTCAA5980.152514448066025No Hit
GTCTGGGACCCCAGAGTCCCGCTTAGAGACCAGAGAAATCAGGCGCCTTGGAGATTGGCCTGGCGCCTGGTGAAA5830.1486888348202217No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCC5800.147923712171061No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5770.14715858952190036No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5670.14460818069136483No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5570.1420577718608293No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCA5560.14180273097777574No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5410.13797711773197244No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5260.13415150448616914No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG5070.12930572770815163No Hit
GTCCAAGGGTGACGGGCAGGGAGGGTGGGGACTGAGTCAACATAACTTCG4970.12675531887761607No Hit
GGCCAAAAGTGGGAGGCCAGTGTGTTGCTTGCATACAATAATAGACTCCA4930.12573515534540186No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4930.12573515534540186No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4870.12420491004708056No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGAATGCATTGGCGAAGTTATGTTGA4870.12420491004708056No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4840.12343978739791989No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4700.11986921503517015No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4470.11400327472493842No Hit
ATAATAGACTCCAACATCCTCAGCCTCCACCCGGGTGATTTTGAGTGTGAAATCAGTGCCGGACCCACTGCCG4450.11349319295883131No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4340.11068774324524222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4340.11068774324524222No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4210.10737221176554601No Hit
AGTCAAAGCCTCGTAAACAGCCATGGAAACAACTACTTGAGTTGGTTTCACCAGGCGCCAGGCCAATCTCCAAGG4130.1053318847011176No Hit
GTGTGTTGCTTGCATACAATAATAGACTCCAACATCCTCAGCCTCCACCC4120.10507684381806404No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT4110.10482180293501049No Hit
GTCCGGCACTGATTTCACACTCAAAATCACCCGGGTGGAGGCTGAGGATG4060.10354659851974271No Hit
GCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGGGAGG4020.10252643498752849No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3960.10099618968920719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)