FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392094
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATTGTATGCAAGCAACACACTGGCCTCCCACTTTTGGCCAGGGGA14890.3797558748667411No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14390.36700383071406345No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTA13220.33716404739679773No Hit
CAATAATAGACTCCAACATCCTCAGCCTCCACCCGGGTGATTTTGAGTGTGAAATCAGTGCCGGACCCACTGCCG11850.3022234464184609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11250.2869209934352477No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10010.25529592393660705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9960.25402071952133926No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9820.2504501471585895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8880.22647630415155548No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7260.18515968109687983No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7100.18107902696802297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6860.17495804577473767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6630.16909210546450598No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC6510.16603161486786333No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6420.16373624692038136No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG6410.16348120603732777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6400.16322616515427424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6220.15863542925931026No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5990.15276948894907852No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5720.1458833851066326No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5690.14511826245747192No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5650.1440980989252577No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5610.1430779353930435No Hit
GTCTGGGACCCCAGAGTCCCGCTTAGAGACCAGAGAAATCAGGCGCCTTGGAGATTGGCCTGGCGCCTGGTGAAA5610.1430779353930435No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5540.14129264921166862No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5430.13848719949807956No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5290.1349166271353298No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCCGGCACTGATTTCACACTCAA5200.1326212591878478No Hit
GTCCAAGGGTGACGGGCAGGGAGGGTGGGGACTGAGTCAACATAACTTCG5170.13185613653868716No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5110.13032589124036584No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCA5100.13007085035731228No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5030.1282855641759374No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5010.1277754824098303No Hit
GTGTGTTGCTTGCATACAATAATAGACTCCAACATCCTCAGCCTCCACCC4830.12318474651486634No Hit
GGTGGAGGCTGAGGATGTTGGAGTCTATTATTGTATGCAAGCAACACACTGGCCTCCCACTTTTGGCCAGGGGAC4670.11910409238600947No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG4660.11884905150295594No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4550.11604360178936682No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGAATGCATTGGCGAAGTTATGTTGA4510.11502343825715262No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCC4470.11400327472493842No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4460.11374823384188484No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4350.11094278412829577No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4330.11043270236218866No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4230.10788229353165311No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4140.10558692558417114No Hit
GCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGGGAGG4090.10431172116890337No Hit
GGCCAAAAGTGGGAGGCCAGTGTGTTGCTTGCATACAATAATAGACTCCA4070.10380163940279627No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4020.10252643498752849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)