FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795544
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28550.3588739277777219No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24680.31022796979174No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAGAGTACTGCTACTTGTATATGAGCTGCAGTAATAAT21600.2715123236426898No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20860.26221051255493094No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAGAGTACTGCTACTTGTATATGAGCTG19050.23945878543487226No Hit
GATTATTACTGCAGCTCATATACAAGTAGCAGTACTCTGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT18110.22764297135042183No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18080.2272658709009181No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17450.2193467614613397No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17410.21884396086200136No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC17350.21808975996299387No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16450.20677674647788183No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15980.2008688394356566No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15870.19948613778747623No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15530.19521233269310057No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15420.1938296310449202No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15000.1885502247518679No Hit
GTACTCTGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC14690.18465352010699596No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGACGTCACCTCCAGGGAGA14670.1844021198073268No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14400.18100821576179318No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14340.18025401486278572No Hit
CCAGAGTACTGCTACTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC13880.1744718079703951No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12980.16315879448528303No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12870.16177609283710265No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATAGTCAGGGACG12790.16077049163842602No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12620.1586335890912382No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12590.15825648864173444No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12210.15347988294802048No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11920.14983457860281768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11810.14845187695463732No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11330.14241826976257754No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11000.13827016481803647No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10750.135127661072172No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10670.13412205987349538No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10660.13399635972366078No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10440.13123095642730004No Hit
GACTATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCT10180.127962752531601No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10050.12632865058375148No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATT9930.12482024878573655No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGTAATTATAATTATGTCTCCTGGTACCAACAATACCCAGGCAAAGC9670.12155204489003751No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC9190.11551843769797773No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9150.11501563709863942No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9010.11325583500095532No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAGAGTACTGCTAC8910.11199883350260953No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8910.11199883350260953No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8830.11099323230393292No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8820.11086753215409832No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8800.11061613185442917No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC8750.10998763110525626No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8720.10961053065575255No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8600.1081021288577376No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8210.10319982301418902No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8160.10257132226501614No Hit
GTATATTACTGTGCGAGCATGTCGACTGGGCCTTACGGTTTTTGGAGTCACGTCCGGGACTGGGGCCAGGGAACC8160.10257132226501614No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8070.10144002091650493No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7960.10005731926832458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC159.096222E-487.6872569
CCTAACG150.002477760268.1234052
CTAACGC150.002477760268.1234053
ATCTCGT800.060.17542337
TCGCGTA200.00742711551.62808238
CACGATA200.007743548651.08613216
TGCCGTC950.047.47325545
TCGTATG950.047.18923640
CGCGTAT306.3560746E-445.93247639
GATCTCG1050.045.8072436
CCGTCTT1150.045.37243747
ACGAGAC2450.044.4859622
CGTATGC1100.043.9564341
GGTATCA8000.043.0056041
ATGCCGT1050.042.91077444
GACATCT2650.042.4404726
CACGAGA2650.041.12852521
GTATGCC1300.039.9193142
CGAGCCC2850.039.4324315
ATCGCGT350.001369672439.29823737
Produced by FastQC (version 0.11.5)