FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795544
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27280.34291000874873045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24050.30230886035216153No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22930.2882304435706887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22130.27817443158392247No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19980.25114889936948803No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAGAGTACTGCTACTTGTATATGAGCTGCAGTAATAAT19750.24825779592329275No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAGAGTACTGCTACTTGTATATGAGCTG19420.24410969097875163No Hit
GATTATTACTGCAGCTCATATACAAGTAGCAGTACTCTGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT18580.23355087839264707No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18250.22940277344810595No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18030.2266373701517452No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG17650.22186076445803124No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17130.21532435666663316No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC16330.20526834467986685No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15970.20074313928582205No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15890.19973753808714542No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15170.19068712729905574No Hit
GTACTCTGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC14990.18842452460203332No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14380.18075681546212405No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGACGTCACCTCCAGGGAGA13880.1744718079703951No Hit
CCAGAGTACTGCTACTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC13070.16429009583379423No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12830.16127329223776435No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12650.15901068954074193No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12460.15662238669388492No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12280.15435978399686254No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12110.1522228814496747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12100.15209718129984012No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11490.14442947215993082No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGTAATTATAATTATGTCTCCTGGTACCAACAATACCCAGGCAAAGC10920.13726456361935982No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10880.13676176302002152No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10870.13663606287018695No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10810.13588186197117946No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10790.1356304616715103No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10580.13299075852498415No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10550.13261365807548042No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATAGTCAGGGACG10330.1298482547791197No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9970.12532304938507485No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9820.12343754713755618No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9580.12042074354152629No Hit
GACTATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCT9560.12016934324185714No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9410.11828384099433846No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9390.11803244069466931No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9230.11602123829731605No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATT9070.1140100358999628No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9070.1140100358999628No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8950.11250163410194784No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8640.10860492945707592No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8610.10822782900757218No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8200.10307412286435445No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8130.10219422181551241No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC8060.10131432076667035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG200.00687687752.6428552
GTTCCGA200.007820521550.9584667
CCGAAAC454.948995E-543.19996368
GGTATCA8600.042.7139171
GCCGTTA603.2813477E-739.63685215
CCGTTAA656.1353603E-736.5947716
GTATCAA20950.036.5294041
GTGAGCG2401.8189894E-1235.71447870
GCGCGGT400.002788432934.00009525
AGGTTAC400.00279963233.972317
TATCAAC23850.031.7764512
AATGGTA4050.031.74620470
TGGTATC2400.031.1530322
GCACTTA553.56408E-430.8839196
TTTTGAT7650.030.81249470
TCAACGC24800.030.5476824
ATCAACG24800.030.4126073
GGTACCG902.2822678E-730.1976096
AACGCCA450.00498373230.1976095
GGATGGG4750.030.07535270
Produced by FastQC (version 0.11.5)