FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882035
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58270.3096116703461944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55590.2953717651372052No Hit
GAGTTACAGTACCCCTTACAGTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAACTGTGGCTGCACCAT51430.2732680316784757No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46870.24903893923332987No Hit
ATTATGACCCATGTAAATTGGTATCAGAAACAGCCAACGACAGCCCCTAA46170.24531956100710137No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42390.22523491858546732No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42380.22518178461080693No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38040.20212163960819005No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37070.19696764406613052No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36180.19223872032135428No Hit
GGTCATAATGGGGTGACTTGACCGGCAAGTGATGGTGACTCTGTCTCCTACAGAGGCAGACAGGGAGGACGGAGA34740.1845874279702556No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCACCCCATTATGACCCATGTAAATTGGTATCAGAAACAG34290.18219639911053728No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34150.18145252346529156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33200.17640479587255287No Hit
CTGTAACTCTGTTGACAGTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGCC32330.17178214007709738No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30850.16391831182735708No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30600.1625899624608469No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTACAGTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAA30140.16014579962646816No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29490.15669209127354167No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTTTCATCCAGCTGACCCAGTC28520.15153809573148214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27820.14781871750525363No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27500.1461184303161206No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26100.13867967386366353No Hit
GTGCAGCCACAGTTCGTTTGATTTCAAGCTTGGTCCCCTGGCCAAAACTG25990.13809520014239904No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25920.1377232623197762No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25890.137563860395795No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAAGCTTGGTCCCCTGGCCAAAACTGTAAGGGGTA25540.13570417128268072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25120.1334725443469436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24830.13193165908179177No Hit
GTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAACTGTGGCTGCACCA24710.1312940513858669No Hit
GGCCAAAACTGTAAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTAGCA24470.13001883599401712No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23910.1270433334130343No Hit
GTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCA23890.12693706546371347No Hit
CGTCCATACCGTACGGAATGGAGACAAAGGGTGTATCAGGTGGTAATATA22960.12199560582029559No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTTTCAT22910.12172993594699354No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCTACTTACTACTGTCAACAGAGTTACAGTACC22820.12125173017504987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22400.11902010323931275No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22220.11806369169542544No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22120.11753235194882136No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21990.11684161027823606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21710.11535385898774465No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21630.1149287871904614No Hit
GTATGGACGTCTGGGGCCTAGGGACTACGGTCATCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG21370.11354730384929079No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21270.11301596410268672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20850.1107843371669496No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20620.10956225574976024No Hit
GTCTCCTACAGAGGCAGACAGGGAGGACGGAGACTGGGTCAGCTGGATGA20130.10695869099140026No Hit
GAGTGAATTCTGCCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA19400.1030799108411905No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19290.10249543711992604No Hit
ACAGTACCCCTTACAGTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGA19290.10249543711992604No Hit
GTCTGGGGCCGAGGTGAAGAAGCCTGGGTCCTCGTTGAGGGTCTCTTGCA19240.102229767246624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)