FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882035
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79040.41997093571586075No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58950.3132247806231021No Hit
GAGTTACAGTACCCCTTACAGTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAACTGTGGCTGCACCAT51200.27204595026128636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46280.24590403472836583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45350.24096257508494792No Hit
ATTATGACCCATGTAAATTGGTATCAGAAACAGCCAACGACAGCCCCTAA43840.23293934491122642No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43560.231451593620735No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43480.23102652182345174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36900.19606436649690362No Hit
GGTCATAATGGGGTGACTTGACCGGCAAGTGATGGTGACTCTGTCTCCTACAGAGGCAGACAGGGAGGACGGAGA35260.18735039465259679No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35160.1868190549059927No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34230.18187759526257483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32600.1732167573929284No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCACCCCATTATGACCCATGTAAATTGGTATCAGAAACAG31530.1675314221042648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30310.16104907719569508No Hit
CTGTAACTCTGTTGACAGTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGCC29770.15817984256403309No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29130.154779268185767No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTACAGTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAA28540.15164436368080297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28420.1510067559848781No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28310.1504222822636136No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27570.14649036813874344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26800.14239905208989206No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26230.13937041553424884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26080.13857340591434272No Hit
GTTTTGGCCAGGGGACCAAGCTTGAAATCAAACGAACTGTGGCTGCACCA26070.1385202719396823No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTTTCATCCAGCTGACCCAGTC25930.1377763962944366No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25480.13538536743471827No Hit
GTGCAGCCACAGTTCGTTTGATTTCAAGCTTGGTCCCCTGGCCAAAACTG25440.13517283153607662No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25430.13511969756141623No Hit
GTAGTAAGTAGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGCCCCAGATCCACTGCCA24710.1312940513858669No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAAGCTTGGTCCCCTGGCCAAAACTGTAAGGGGTA24630.13086897958858365No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24420.12975316612071508No Hit
GGCCAAAACTGTAAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTAGCA23510.12491797442661799No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23440.12454603660399514No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCTACTTACTACTGTCAACAGAGTTACAGTACC22610.12013591670718131No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22580.1199765147832001No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22050.11716041412619851No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTTTCAT21400.11370670577327202No Hit
GTATGGACGTCTGGGGCCTAGGGACTACGGTCATCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG20780.11041239934432676No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20520.10903091600315616No Hit
GTCTGGGGCCGAGGTGAAGAAGCCTGGGTCCTCGTTGAGGGTCTCTTGCA20300.10786196856062719No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20080.10669302111809822No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20040.10648048521945659No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19900.1057366095742109No Hit
GAGTGAATTCTGCCCCAGATCCACTGCCACTGAACCTTGATGGGACTCCA19880.10563034162489007No Hit
CGTCCATACCGTACGGAATGGAGACAAAGGGTGTATCAGGTGGTAATATA19590.10408945635973826No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19510.10366438456245501No Hit
GTCTCCTACAGAGGCAGACAGGGAGGACGGAGACTGGGTCAGCTGGATGA19320.10265483904390725No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19320.10265483904390725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5400.043.2315441
GTATCAA48100.037.9130821
ATCAACG54950.032.8624273
TCAACGC55400.032.6513254
TACGTAT900.003676277632.13534570
CAACGCA56550.031.990735
AACGCAG56700.031.9060966
ACGCAGA59550.030.265547
CGCAGAG59650.030.1581128
CGGGTAT450.005088329330.07171817
AGAGTAC60200.029.54398711
GCAGAGT62500.028.8911089
TATCAAC64050.028.0871052
GTACCGT500.0085319127.0515986
AATGGTA8250.025.41613670
CAGAGTA72100.025.23190110
TGTTAGA21200.024.89731470
TCTCGCG10450.024.2168370
AGTACGG48300.024.21678513
GAGTACG48500.024.1898412
Produced by FastQC (version 0.11.5)