FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745728
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG103700.5940215199618727No Hit
GATTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA97910.5608548410748982No Hit
CTACTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCA68250.39095437548117457No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC67660.38757469663086114No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT62410.3575012831323093No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC59240.33934266964842175No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57720.33063570040693624No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT51000.2921417311287898No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC47850.27409768302965865No Hit
CTACAGTAGTGATCAGCGGCCCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCC45610.2612663599369432No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43110.2469456868423947No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42710.24465437914726693No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39400.2256938079700847No Hit
GTAGTGATCAGCGGCCCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAG39320.22523554643105914No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCAC39150.22426174066062982No Hit
GATCACTACTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGCAAATAGTATTACTTCCGA37950.21738781757524653No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37820.21664314257433004No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36800.2108003079517542No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC36700.21022748102797228No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCCCAGG35690.20444192909777467No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG35540.20358268871210178No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34700.19877094255233346No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34190.19584952524104557No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33810.1936727829306742No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG33710.19309995600689225No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32940.1886891886937713No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32760.18765810023096383No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32270.1848512483044323No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC32080.18376287714924663No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31720.18170070022363163No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31540.18066961176082413No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30350.17385297136781905No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGATCAGGGACC29530.16915579059280714No Hit
CCCCTGAGGGCCGCTGATCACTACTGTAGATGAGGAGTTTGGGGGCCGTT28310.16216730212266744No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28280.16199545404553287No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28220.1616517578912637No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT28130.16113621365985994No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28110.16102164827510357No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27720.158787623272354No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCGGAGGGAC27600.15810023096381567No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27550.1578138175019247No Hit
CTCTTGTTCTGGAAGCAGCTCCAACGTCGGAAGTAATACTATTTGCTGGTACCAGCAGGTCCCAGGAACGGCCCC27510.15758468673241194No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC27410.15701185980862997No Hit
GTATTACTTCCGACGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCAT26650.15265837518788722No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25960.14870586941379185No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC25910.14841945595190087No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCACCATCTCTTGTTCTGG25510.14612814825677312No Hit
ATACCACACCTCCCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCC25480.1459563001796385No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC25170.14418053671591452No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC24980.14309216556072882No Hit
CCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24360.13954063863328078No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGATCAGGGACCCCTGAGGGCCGCTGATCA24040.1377075924771786No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT23460.1343851963192433No Hit
CCTCCCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCCTC23340.13369780401070497No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCCCAGGC23300.13346867324119221No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC23220.13301041170216665No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA23050.13203660593173736No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAT22820.1307191040070389No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG22210.12722485977196904No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA22170.1269957290024563No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCG21910.12550637900062322No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC21880.12533453092348865No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21600.12373061553689922No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC21570.12355876745976464No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC21430.12275680976646991No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21150.12115289437988049No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG20890.11966354437804744No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20880.11960626168566924No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20740.11880430399237453No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20500.11742951937529786No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20480.11731495399054147No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19870.11382070975547165No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19780.11330516552406789No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19730.11301875206217692No Hit
GCTTGGTCCCTCCGCCGAATACCACACCTCCCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCCT19690.11278962129266416No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC19510.11175853282985666No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19420.11124298859845291No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19270.11038374821278No Hit
GTGCCAGACTTGGAGGCAGAGAATCGATCAGGGACCCCTGAGGGCCGCTG19240.11021190013564541No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18990.10877983282619055No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18750.10740504820911391No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18710.10717591743960113No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATG18610.10660309051581918No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGC18420.10551471936063349No Hit
GAAGTAATACTATTTGCTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAA18250.1045409135902042No Hit
GTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGCAAATAGTATTACTT17810.10202047512556367No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17580.1007029732008652No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17510.10030199435421784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG5400.056.01756370
CGCGTAT408.494044E-751.54797439
ATCGCGT451.966424E-645.63672337
TCGTATG4850.044.0246640
CGTATGC4800.043.85294341
GGTATCA17450.043.1366161
ATGCCGG1250.041.6489944
CTTATAC8850.041.1924061
TATGCCG5300.041.16754543
GTATGCC5350.040.7205642
TCTCGTA3300.040.530838
GTCGTAT1700.040.42978339
TGTCGTA1450.040.20827538
TATACAC9250.040.1374933
GCCGTCT3850.039.83468646
TTATACA9450.039.6451872
TAGGCAT458.3386374E-539.54594455
ACGAGAC9100.039.315522
ATGCCGT4450.038.9971844
CCCTAAG9050.038.8547128
Produced by FastQC (version 0.11.5)