FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745728
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA93680.5366242621989221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81960.4694889467316787No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC78990.452475987095355No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG64830.37136369468783226No Hit
CTACTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCA63170.36185476775305203No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62080.3556109542838289No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT59170.3389416908017744No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57770.3309221138688272No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC51510.29506314844007775No Hit
CTACAGTAGTGATCAGCGGCCCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCC49160.28160171573120213No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCAC43880.25135645415551566No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC43860.25124188877075926No Hit
GTAGTGATCAGCGGCCCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAG42940.24597188107196538No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42740.2448262272244015No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40600.23256773105546796No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40550.23228131759357704No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39930.22872979066612897No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC39640.22706859258716133No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG39340.22535011181581555No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38270.21922086373134878No Hit
GATCACTACTGTAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGCAAATAGTATTACTTCCGA36490.2090245444880302No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35740.20472834255966565No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35460.20312442717307622No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34650.1984845290904425No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34520.19773985408952596No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32910.18851734061663672No Hit
CTCTTGTTCTGGAAGCAGCTCCAACGTCGGAAGTAATACTATTTGCTGGTACCAGCAGGTCCCAGGAACGGCCCC32540.18639788099864354No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT32390.18553864061297062No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32020.18341918099497745No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA31150.17843558675807455No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30970.17740449829526705No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCGGAGGGAC29910.1713325329031785No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCCCAGG29550.1692703559775635No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29360.16818198482237784No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29160.16703633097481393No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29050.1664062213586538No Hit
CCCCTGAGGGCCGCTGATCACTACTGTAGATGAGGAGTTTGGGGGCCGTT28660.16417219635590424No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC28300.16211001943028924No Hit
GTATTACTTCCGACGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCAT27790.15918860211900135No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27570.15792838288668107No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27490.15747012134765553No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26750.15323120211166918No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26190.1500233713384903No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT25480.1459563001796385No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCACCATCTCTTGTTCTGG25250.14463879825494005No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC25070.14360770979213255No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25020.1433212963302416No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC24950.14292031748359424No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24800.14206107709792132No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA24750.14177466363603036No Hit
CCTCAGGGGTCCCTGATCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24660.14125911940462663No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24290.13913965978663342No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23560.13495802324302525No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22750.13031812516039154No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22250.12745399054148182No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC22230.12733942515672544No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC22070.12642290207867435No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22060.12636561938629615No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC22050.12630833669391794No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21990.1259646405396488No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG21890.12539181361586685No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG21880.12533453092348865No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21760.12464713861495033No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC21690.12424615976830296No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAT21350.12229854822744438No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGGCAGAGAATCGATCAGGGACCCCTGAGGGCCGCTGATCA21290.12195485207317519No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGGGAGGTGTGGTATTCGGCG21070.12069463284085492No Hit
ATACCACACCTCCCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCC20470.1172576712981633No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19700.11284690398504235No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19670.11267505590790777No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATG19550.11198766359936943No Hit
GTAATACTATTTGCTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAACTCCTCATCTACAGTAGTGATCAGCGGC19480.11158668475272208No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19430.1113002712908311No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19430.1113002712908311No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCCCAGGC19310.11061287898229279No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC18970.10866526744143416No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCAC18860.10803515782527404No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG18850.10797787513289585No Hit
GAAGTAATACTATTTGCTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAA18830.10786330974813946No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18790.10763417897862668No Hit
GTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGCAAATAGTATTACTT18730.10729048282435752No Hit
GATCGATTCTCTGCCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGG18450.10568656743776808No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18250.1045409135902042No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18220.10436906551306961No Hit
GCTTGGTCCCTCCGCCGAATACCACACCTCCCAGGCTGTCATCCCATGATGCACAGTAATAATCAGCCTCATCCT18120.10379623858928769No Hit
GTGCCAGACTTGGAGGCAGAGAATCGATCAGGGACCCCTGAGGGCCGCTG18100.1036816732045313No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18020.10322341166550573No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18000.10310884628074936No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17920.1026505847417238No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC17820.10207775781794184No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17720.10150493089415992No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGATGGTCACCATCTCTTGTTCTGGAAGCAG17510.10030199435421784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.044.9139021
CCTAACG651.3942554E-841.49972
GTATCAA40450.038.537011
CTAACGC656.5251515E-736.3122373
TTGCCGA400.002191517535.71392456
TACTGAA5550.034.9055470
GGCAGAG35950.033.7833570
TATCAAC45750.033.7553562
TGGTATC4800.033.7185022
CGACGGT500.003756830731.9948268
ATCAACG48350.031.8006973
TCAACGC48850.031.5433254
CAACGCA50300.030.6340275
AATGGTA7100.030.43362670
AACGCAG50950.030.3102556
TATGCGG450.005172260629.9711425
TCTCGCG5000.029.80396570
ACGTTGG35400.029.67767370
GTCCTAG803.2858352E-629.5231461
CGAGTTC500.00616595628.91246460
Produced by FastQC (version 0.11.5)