FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547930
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGTTCACCCTACACTTTTGGCCAGGGGA56520.3651327902424528No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46620.3011764097859722No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46410.29981975929144083No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGATTAGACTGAAGTCTGTCACGGACCCATAGCCA41920.2708132796702693No Hit
GTATGGAAGTTCACCCTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT34780.2246871628562015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32230.20821354970832015No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA31570.20394979101122143No Hit
GTGTATTACTGTCAGCAGTATGGAAGTTCACCCTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA29980.19367800869548366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29710.19193374377394326No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG28900.18670094900932213No Hit
GTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGATTAGACTGAAGTCTGTCACGGACCCATAGCCA27830.1797884917276621No Hit
CTCCTGCAGGGCCAGTGAGGCAGTTGGCAGCAATTACTTAGCCTGGTTCC26430.17074415509745275No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26390.17048574547944675No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAATTGCTGCCAACTGCCTCA25440.16434851705180467No Hit
CTTCCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCT25020.16163521606274187No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24490.1582112886241626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23330.1507174097019891No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22010.1421898923077917No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCCGTGACAGACTTCAGT21840.14109165143126626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21600.13954119372323037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21530.13908897689171992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20940.13527743502613165No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20600.13308095327308084No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20600.13308095327308084No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19970.1290110017894866No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGGCAGTTGGCAG19940.12881719457598212No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG19900.12855878495797612No Hit
GGCTATGGGTCCGTGACAGACTTCAGTCTAATCATCAGCAGACTGGAGCCTGAAGACTTTGCAGTGTATTA19890.12849418255347464No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19390.12526406232839987No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC19280.12455343587888341No Hit
CCCATAGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCAC18690.12074189401329517No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18550.11983746035027425No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCCGCTCACCTCTATGTAGG18440.1191268339007578No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18260.11796399061973087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17950.1159613160801845No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17490.1129896054731157No Hit
GTCTATTACTGTGCGAGAGACCCAGGGCGTGCCCCTGACAGCTGGGGCCA16990.10975948524804092No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16890.10911346120302597No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16760.10827362994450652No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16290.10523731693293625No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGG16230.10484970250592728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16200.10465589529242278No Hit
CCTCTATGTAGGCAGTACTCGTGGATTTGTCCGCAGTAATCGTTATTCTGCCCTGGAACTTCTGTGCGTAGTCTC16170.1044620880789183No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15710.1014903774718495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)