FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547930
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGTTCACCCTACACTTTTGGCCAGGGGA53360.34471843041998024No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47800.30879949351714875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44700.2887727481216851No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGATTAGACTGAAGTCTGTCACGGACCCATAGCCA40790.2635132079616003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38710.25007590782528927No Hit
GTATGGAAGTTCACCCTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT35630.23017836723882865No Hit
GTGTATTACTGTCAGCAGTATGGAAGTTCACCCTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAA32570.210410031461371No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32320.2087949713488336No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA29690.19180453896494026No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG28180.18204957588521445No Hit
GTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGATTAGACTGAAGTCTGTCACGGACCCATAGCCA28080.1814035518401995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27400.1770105883340978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26000.1679662517038884No Hit
CTCCTGCAGGGCCAGTGAGGCAGTTGGCAGCAATTACTTAGCCTGGTTCC25020.16163521606274187No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24950.1611829992312314No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAATTGCTGCCAACTGCCTCA24070.15549798763509978No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23590.15239707221902798No Hit
CTTCCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCT23460.15155724096050854No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21980.1419960850942872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21010.13572965185764213No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20500.13243492922806588No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG20430.1319827123965554No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20070.12965702583450156No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19980.12907560419398809No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19910.12862338736247764No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19240.12429502626087743No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCCGTGACAGACTTCAGT19120.12351979740685948No Hit
GGCTATGGGTCCGTGACAGACTTCAGTCTAATCATCAGCAGACTGGAGCCTGAAGACTTTGCAGTGTATTA19070.123196785384352No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18940.12235695412583256No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGGCAGTTGGCAG18870.12190473729432211No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC18830.12164632767631611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18510.11957905073226825No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18150.11725336417021442No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18060.11667194252970095No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18030.11647813531619647No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCCGCTCACCTCTATGTAGG17880.11550909924867403No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGG17200.11111613574257234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16900.10917806360752748No Hit
GTCTATTACTGTGCGAGAGACCCAGGGCGTGCCCCTGACAGCTGGGGCCA16780.10840283475350952No Hit
CCCATAGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCAC16280.10517271452843474No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15640.10103816064033902No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA15500.1001337269773181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)