FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387862
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48370.34852168299153663No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42710.30773953029912193No Hit
AGCCAGGACAGGCCCCTGTCCTTATCTTCTATGGTAAAAACAGTCGGCCC42000.3026237478942431No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40120.28907773251231034No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC38240.2755317171303775No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36820.26530015232061976No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36520.26313855412137516No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35530.256005280063868No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAACCA33300.2399374001161499No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33120.2386404411966031No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAACCAG30060.21659213956430828No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30050.2165200862910001No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29830.21493491427822076No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAG27960.20146095216959614No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG27690.19951551379027596No Hit
CTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT27060.19497615757186232No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTAC25190.1815021954632377No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTGTTGAGGCCGTCTCCTTGGCATGTAATCCTGACTGT25120.18099782255008062No Hit
CTATTACTGTAACTCCCGGGACAGCACTGGTTACCATCTAGTCTTCGGAA24040.1732160690328001No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22690.16348887713619942No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22510.1621919182166527No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21910.15786872181816347No Hit
GACTATTACTGTAACTCCCGGGACAGCACTGGTTACCATCTAGTCTTCGG21690.15628354980538411No Hit
GATAAGGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAAT20920.15073544776065634No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20710.1492223290211851No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20700.14915027574787693No Hit
GGATTACATGCCAAGGAGACGGCCTCAACAAATATTATGGAAGCTGGTACCAGCAGAAGCCAGGACAGGCCC20520.1478533168283302No Hit
CTCCTCACTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA20480.14756510373509757No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGA20310.14634019808885898No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20300.14626814481555084No Hit
GTCCTTATCTTCTATGGTAAAAACAGTCGGCCCTCAGGGATCCCAGATCG20190.14547555880916116No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGA19150.1379820183851132No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18820.13560426036594417No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18770.1352439939994034No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAAC18610.13409114162647295No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGAAG18510.13337060889339142No Hit
GCACTGGTTACCATCTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18190.13106490414753053No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18030.12991205177460008No Hit
GTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC17850.12861509285505332No Hit
GTTATGATCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGATC16750.12068923279115647No Hit
GGACAGCACTGGTTACCATCTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC16090.11593371675281837No Hit
AACCAGTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGAAG15630.11261926618064333No Hit
GTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAAGGAGACGGCCTCAACAAATATTATGGAAGCTGGTA15460.11139436053440471No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15420.1111061474411721No Hit
GCCCTCAGGGATCCCAGATCGATTCTCTGGCTCCAGCTCAGGAAACACAG15090.10872838942200305No Hit
GGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTG14870.1071432174092237No Hit
GATCATAACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACT14770.10642268467614216No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14670.10570215194306062No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14610.1052698323032117No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14260.10274796773742634No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14180.10217154155096111No Hit
GTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGAAGCTGTGTTTC14090.10152306209118775No Hit
GGTTACCATCTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACT14010.10094663590472253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)