FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387862
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT44280.319051894208502No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC38580.27798152842285473No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38420.2768286760499243No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38080.27437886475744705No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37090.2672455906999399No Hit
AGCCAGGACAGGCCCCTGTCCTTATCTTCTATGGTAAAAACAGTCGGCCC36050.259752050275892No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC35290.2542760015044723No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32630.23510983080450362No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32320.23287617933195084No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32180.23186743350563674No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29190.21032350478649894No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAACCA27600.1988670343305026No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAACCAG26970.19432767811208895No Hit
CTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT26840.19339098555908296No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAG25920.18676208441473288No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24500.17653051960497512No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTGTTGAGGCCGTCTCCTTGGCATGTAATCCTGACTGT24280.17494534759219577No Hit
CTATTACTGTAACTCCCGGGACAGCACTGGTTACCATCTAGTCTTCGGAA23960.17263964284633487No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23800.1714867904734044No Hit
CTCCTCACTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGA22890.1649299426023625No Hit
GTCCTTATCTTCTATGGTAAAAACAGTCGGCCCTCAGGGATCCCAGATCG22220.16010237329071622No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCCTGGGACAGACAGTCAGGATTAC22170.15974210692417545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21980.15837309473132055No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21640.15592328343884335No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21180.1526088328666683No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20750.14951054211441772No Hit
GGATTACATGCCAAGGAGACGGCCTCAACAAATATTATGGAAGCTGGTACCAGCAGAAGCCAGGACAGGCCC20710.1492223290211851No Hit
GATAAGGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAAT20680.14900616920126064No Hit
GACTATTACTGTAACTCCCGGGACAGCACTGGTTACCATCTAGTCTTCGG20590.14835768974148725No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20090.14475502607607962No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAGATGGTAAC19790.14259342787683502No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGA19230.13855844457157845No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18920.1363247930990257No Hit
GTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGC18820.13560426036594417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18550.13365882198662404No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18060.13012821159452453No Hit
GCACTGGTTACCATCTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA17700.12753429375543102No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGA17510.1261652815625761No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17370.12515653573626195No Hit
GGACAGCACTGGTTACCATCTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC17250.12429189645656413No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16740.12061717951784832No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGAAG16720.12047307297123201No Hit
GTTATGATCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCAGAGAATCGATC15850.11420443819342269No Hit
GTCTGTGGCCCTGGGACAGACAGTCAGGATTACATGCCAAGGAGACGGCCTCAACAAATATTATGGAAGCTGGTA15700.11312363909380038No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15360.11067382780132319No Hit
GGACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTCCATAATATTTG15230.1097371352483172No Hit
AACCAGTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTTATGATCAAGGAAG15020.10822401650884597No Hit
CCCCTGTCCTTATCTTCTATGGTAAAAACAGTCGGCCCTCAGGGATCCCA14850.10699911086260738No Hit
GCCCTCAGGGATCCCAGATCGATTCTCTGGCTCCAGCTCAGGAAACACAG14520.10462135284343833No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14280.10289207428404265No Hit
GGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGC14130.10181127518442035No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14010.10094663590472253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA7000.035.79454870
GTATCAA33600.034.807511
CCGAAAC701.37473035E-534.38093668
CGCAATA450.00388933231.76884557
TATCAAC36550.031.2459142
TAGTACT652.685361E-531.175544
GGTATCA19650.030.6191831
GTGGTAT4250.030.2225321
CCTAACG450.00511929630.0338762
CGTAGTA450.00511929630.0338762
CTAACGC450.00512200330.0306263
ATCAACG38600.029.4082323
TCAACGC38800.029.3342024
CAACGCA38900.029.0872485
TAGATTA854.1302883E-628.6677346
TACGTAT1504.7922542E-528.63563570
AACGCAG39750.028.3802836
TAGGCAA3550.028.23231970
TGGTATC4750.027.0304892
CGCAGAG42100.026.7168458
Produced by FastQC (version 0.11.5)