FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555493
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTACTACCTGCATATGAG21540.38776366218836245No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20800.3744421621874623No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18560.33411762164419717No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTCGTGGTATTCGG17120.30819470272352667No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16910.30441427704759555No Hit
GACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTA16480.29667340542545095No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTC15600.2808316216405967No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14290.2572489662335979No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12910.23240616893462204No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG12720.2289857837992558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12680.22826570271812607No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12370.22268507433937063No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA11890.21404410136581378No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11590.20864349325734077No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11570.2082834527167759No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11460.20630322974366913No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATTACCTTGTCTCCTGGTACCAACAGCA11350.20432300677056237No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11240.2023427837974556No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11190.2014426824460434No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11190.2014426824460434No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATTACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC11060.1991024189323718No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG11050.19892239866208936No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10590.1906414662290974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10350.18632097974231898No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9810.17659988514706756No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9790.17623984460650272No Hit
ACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTAC9000.16201824325419042No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8860.15949795947023637No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8810.15859785811882418No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8800.15841783784854174No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8560.15409735136176334No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8530.15355729055091602No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8490.1528372094697863No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8300.14941682433442005No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8280.14905678379385517No Hit
GTGTATTACTGTGCGAGATCCCCCGGCATAGGATTTTGTAGTAGTGCCAGCTGCTATACGGGTTTGGCCTTTGA8000.14401621622594704No Hit
GTTATTACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA7620.13717544595521458No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7490.1348351824415429No Hit
CCCCTGAGGGCCGCTTACTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA7380.13285495946843615No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7380.13285495946843615No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCGTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7020.12637422973826853No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6870.12367392568403202No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6780.12205374325149011No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6720.12097362162979551No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6660.11989350000810091No Hit
CTGCTGCTCCTCACCCTCGTCACTCAGGACACAGGGTCCTGGGCCCAGTC6570.118273317575559No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCA6570.118273317575559No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC6530.11755323649442927No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6510.1171931959538644No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6400.11521297298075764No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTG6310.11359279054821572No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6260.11269268919680356No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA6140.11053244595341435No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG6140.11053244595341435No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6120.11017240541284948No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT6090.10963234460200219No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6020.10837220271002516No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6000.10801216216946027No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5970.10747210135861299No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5940.10693204054776567No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTC5900.10621195946663593No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5820.10477179730437648No Hit
GTGCTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC5770.1038716959529643No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5770.1038716959529643No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5690.10243153379070483No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC5690.10243153379070483No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5620.1011713918987278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGC208.452916E-569.0942242
CGTATGC454.3655746E-1061.35006341
TCGTATG454.4019544E-1061.29989640
ATCTCGT401.2227247E-860.194437
GACATCT900.056.98707226
CGAGACA1050.055.3239723
ACGAGAC1000.054.66818222
CACGAGA950.053.95372421
CCCACGA950.053.94886419
GCCCACG950.053.94400818
CGAGCCC950.053.94400815
GATATAG200.006399512353.6069264
GTAGGTA200.00759792751.3299928
ATGCACG200.007646807551.24680316
GGTATCA4450.050.675561
ATCTCCG1100.049.68492510
TCCGAGC1100.049.68492513
CCGTCTT605.2641553E-946.324347
TAGTCAC306.379556E-445.8956838
TACGGAT4750.045.8783270
Produced by FastQC (version 0.11.5)