FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555493
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20190.3634609257002338No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTACTACCTGCATATGAG19440.3499594054290513No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTCGTGGTATTCGG17880.32187624326499165No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17430.3137753311022821No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17220.309994905426351No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTC14190.25544876353077356No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13530.24356742569213294No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13040.23474643244829366No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT12970.23348629055631667No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12750.22952584461010309No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12740.22934582433982065No Hit
GACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTA12680.22826570271812607No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATTACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC11640.20954359460875296No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11630.20936357433847053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11580.20846347298705833No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11340.20414298650027995No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA11280.20306286487858533No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11110.20000252028378399No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10990.19784227704039473No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATTACCTTGTCTCCTGGTACCAACAGCA10930.19676215541870015No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG10780.19406185136446363No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10040.18074035136356353No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9690.17443964190367836No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9500.17101925676831212No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9480.17065921622774727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9390.16903903379520535No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9340.1681389324437932No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8840.15913791892967147No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8730.1571576959565647No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8450.15211712838865657No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT8260.14869674325329033No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8230.148156682442443No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8150.14671652028018356No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8150.14671652028018356No Hit
CTGCTGCTCCTCACCCTCGTCACTCAGGACACAGGGTCCTGGGCCCAGTC7910.14239603379340512No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7840.1411358919014281No Hit
CCCCTGAGGGCCGCTTACTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA7640.13753548649577943No Hit
ACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTGCTAC7580.13645536487408483No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCGTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC7550.1359153040632375No Hit
GTGTATTACTGTGCGAGATCCCCCGGCATAGGATTTTGTAGTAGTGCCAGCTGCTATACGGGTTTGGCCTTTGA7550.1359153040632375No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7530.13555526352267266No Hit
GTTATTACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA7530.13555526352267266No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCTCTCCGCCGAATACCACGAAAGTG7120.12817443244109286No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7060.12709431081939826No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6980.12565414865713878No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC6530.11755323649442927No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6320.11377281081849816No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6310.11359279054821572No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6230.11215262838595627No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6190.11143254730482653No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6160.11089248649397922No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6090.10963234460200219No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6030.10855222298030759No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT5990.10783214189917785No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG5970.10747210135861299No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT5960.10729208108833053No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG5950.10711206081804811No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA5940.10693204054776567No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCGTCACTCAGGACACAGGGTCCTGGGC5820.10477179730437648No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCA5730.10315161487183457No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC5720.10297159460155214No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC5700.10261155406098726No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5580.10045131081759806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTT251.4740555E-461.7495468
TAATACG200.007697011351.1620454
GGTATCA4850.046.4359551
TGAACTT657.4111193E-444.51164670
TACGGAT5000.043.39885370
GTATCAA15000.042.5404971
GGTACCT350.001420638339.00518419
TATCAAC17350.035.9820982
TGGTATC1650.035.1479382
ATCAACG18200.034.2985233
TCAACGC18250.034.2014774
ATCCAGT400.002732636934.13876326
GCTTGTA400.00273867934.123391
TATACTG400.002742309634.1141745
AACGCAG18750.033.662356
CAACGCA18700.033.5668835
TCTTCGG1352.1704805E-532.147370
ACGCAGA19750.032.1306727
CGGGTAC751.8163246E-631.848517
CGCAGAG20150.031.4928428
Produced by FastQC (version 0.11.5)