FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830223
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT43430.5231124649642326No Hit
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGTCGCTGATGGTGAGAGTGAACTCTGTC41970.5055268283340741No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26630.3207571941514509No Hit
GAGTATTGGTGACTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAGGCCCCTAAACTCCTAATCTATAAGTCGT25600.308350888857572No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25090.3022079609936125No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATACA21580.2599301633416564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20310.24463306846473779No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18450.22222944919617982No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17700.21319573174918066No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16100.1939238011955824No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15740.18958761682102276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15680.18886491942526284No Hit
GAGTTCACTCTCACCATCAGCGACCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATAGTTAT15630.1882626715954629No Hit
GTATATTACTGTGCGAGAGACCCGGGACTCTCGTACTTTGATCTCTGGGG15520.18693772636990302No Hit
GTCTACATTTGTAGGAGACAGAGTCAACATCACTTGCCGGGCCAGTCAGA15360.1850105333145432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14760.17778355935694384No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGTTGACTCTGTCTCCTACAAA14610.17597681586754402No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA14570.17549501760370406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14320.17248377845470433No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13880.1671839975524648No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13210.15911387663314555No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13210.15911387663314555No Hit
CCTTCCACCCTGTCTACATTTGTAGGAGACAGAGTCAACATCACTTGCCG12940.15586173835222583No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12670.15260960007130614No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12300.14815296613078655No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11210.13502396344114773No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11200.13490351387518776No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11170.1345421651773078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11130.13406036691346784No Hit
GGCCAAAAGTGTACGGATAACTATTATAGTGTTGGCAGTAATAAGTTGCA11060.1332172199517479No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10240.1233403555430288No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10120.12189496075150892No Hit
CTCCTGTACAGCCTCTGGATTCAGCTTCACTGTCTATACCATGAACTGGG10070.12129271292170898No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG10000.12044956595998907No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9970.1200882172621091No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9870.11888372160250921No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9820.11828147377270927No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9820.11828147377270927No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9650.11623383115138945No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9410.1133430415683497No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCGACCTGCAGCCTGATGATT9140.11009090328743No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9130.10997045372147002No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9080.10936820589167007No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9080.10936820589167007No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8990.10828415979803017No Hit
CACTATAATAGTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT8970.10804326066611018No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8940.10768191196823022No Hit
GAGTGAACTCTGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACTCCT8720.10503202151711047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)