FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830223
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGTCGCTGATGGTGAGAGTGAACTCTGTC42560.5126333527257135No Hit
CTATAATAGTTATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT40470.4874593934400757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26200.31557786281517136No Hit
GAGTATTGGTGACTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAGGCCCCTAAACTCCTAATCTATAAGTCGT23020.2772749008398948No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21060.25366678591173697No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20930.2521009415542571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19570.2357198005836986No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATACA19350.23306991013257883No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17470.2104253917321009No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15900.1915148098763826No Hit
GAGTTCACTCTCACCATCAGCGACCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATAATAGTTAT15430.18585368027626312No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15290.18416738635282326No Hit
GTATATTACTGTGCGAGAGACCCGGGACTCTCGTACTTTGATCTCTGGGG15130.18224019329746347No Hit
GTCTACATTTGTAGGAGACAGAGTCAACATCACTTGCCGGGCCAGTCAGA14940.17995165154422366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14250.17164063149298442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13870.16706354798650483No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13480.16236601491406527No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA13360.16092062012254538No Hit
GAGTGAACTCTGTCCCAGATGCACTGCCGCTGAACCTTGACGGGACTCCT13300.16019792272678546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13220.15923432619910555No Hit
CCAATACTCTGACTGGCCCGGCAAGTGATGTTGACTCTGTCTCCTACAAA13080.1575480322756657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12270.14779161743290659No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12130.14610532350946673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11900.143334983492387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11640.14020329477742727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11490.13839655128802744No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10980.132253623424068No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10780.1298446321048682No Hit
CCTTCCACCCTGTCTACATTTGTAGGAGACAGAGTCAACATCACTTGCCG10650.12827878774738835No Hit
GGCCAAAAGTGTACGGATAACTATTATAGTGTTGGCAGTAATAAGTTGCA10540.12695384252182848No Hit
CTCCTGTACAGCCTCTGGATTCAGCTTCACTGTCTATACCATGAACTGGG10440.12574934686222858No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10310.12418350250474873No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10300.12406305293878875No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10260.12358125467494879No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9920.11948596943230916No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9910.11936551986634915No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9880.11900417116846919No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG9610.1157520328875495No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9170.11045225198530997No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8970.10804326066611018No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8940.10768191196823022No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8620.10382752585751057No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8570.10322527802771064No Hit
GTCCCAGATGCACTGCCGCTGAACCTTGACGGGACTCCTCTTTGTAAACT8520.10262303019791068No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8510.1025025806319507No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8320.1002140388787109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG6750.047.69098370
GTCTCGT5900.042.70047470
GTGGTAT2400.039.547821
GTATCAA24900.034.7149541
TTAACGG601.425646E-534.1970235
ATAAACG400.00283739733.8797533
GCGTTGT6000.033.0240862
CGTTGTC6300.031.60334263
GGCGTTG6150.031.53383361
GGCACCG652.63033E-531.2679659
CCGTGTC6200.031.14000912
CAGCCGT6150.030.844289
TATACAG6500.030.7468345
CGGCTCT5750.030.66309521
TTGGCGT6300.030.59545759
TTGTCTC6600.030.48075965
GTGAGTT6350.030.41094651
TATCAAC28450.030.0167142
CAGATAC5900.029.9203843
TCAACGC28950.029.602864
Produced by FastQC (version 0.11.5)